catalysis of a SN2 reaction through hydrogen bonding of the liberated oxygen-5' to the histidine, charge neutralization by the two Mg2+ ions, and stabilization of the product sulfonium cation through a close, non-bonded, contact between the sulfur and the ribose oxygen-4'
Cloned(Commentary) (protein specific)
expression in Escherichia coli
Crystallization (Commentary) (protein specific)
quantum mechanical/molecular mechanical calculations, exploiting structures of the active crystalline enzyme, based on PDB files 1P7L and 1RG9