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Literature summary for 2.5.1.6 extracted from

  • Taylor, J.C.; Markham, G.D.
    Conformational dynamics of the active site loop of S-adenosylmethionine synthetase illuminated by site-directed spin labeling (2003), Arch. Biochem. Biophys., 415, 164-171.
    View publication on PubMed
Search for more literature for 2.5.1.6

Protein Variants

Protein Variants Comment Organism
D107C enzyme activity similar to wild-type, attachment of methanethiosulfonate spin label to form D107R1, increase in Km-value, decrease in kcat value Escherichia coli
G105C enzyme activity similar to wild-type, attachment of methanethiosulfonate spin label to form G105R1, increase in Km-value Escherichia coli
additional information mutants D107R1, D105R1, derived from mutants D107C, D105C, by addition of methanethiosulfonate spin label Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
 ATP wild-type, pH 8.0 Escherichia coli
0.11
-
 L-methionine wild-type, pH 8.0 Escherichia coli
0.13
-
 ATP mutant D107C, pH 8.0 Escherichia coli
0.13
-
 ATP mutant G105C, pH 8.0 Escherichia coli
0.18
-
 L-methionine mutant D107R1, pH 8.0 Escherichia coli
0.18
-
 ATP mutant G105R1, pH 8.0 Escherichia coli
0.19
-
 L-methionine mutant D107R1, pH 8.0 Escherichia coli
0.29
-
 L-methionine mutant D107C, pH 8.0 Escherichia coli
0.45
-
 L-methionine mutant G105C, pH 8.0 Escherichia coli
0.75
-
 L-methionine mutant G105R1, pH 8.0 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A817
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
 Escherichia coli phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
 L-methionine mutant D107R, pH 8.0 Escherichia coli
1.4
-
 L-methionine mutant G105R, pH 8.0 Escherichia coli
1.5
-
 L-methionine wild-type, pH 8.0 Escherichia coli
1.6
-
 L-methionine mutant D107C, pH 8.0 Escherichia coli
1.6
-
 L-methionine mutant G105C, pH 8.0 Escherichia coli