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Literature summary for 2.5.1.6 extracted from

  • Reguera, R.M.; Balana-Fouce, R.; Perez-Pertejo, Y.; Fernandez, F.J.; Garcia-Estrada, C.; Cubria, J.C.; Ordonez, C.; Ordonez, D.
    Cloning expression and characterization of methionine adenosyltransferase in Leishmania infantum promastigotes (2002), J. Biol. Chem., 277, 3158-3167.
    View publication on PubMed
Search for more literature for 2.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
His6-tagged fusion protein expressed in Escherichia coli Leishmania infantum

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Leishmania infantum
diphosphate inhibition for S-adenosylmethionine and L-methonine Leishmania infantum
S-adenosyl-L-ethionine
-
 Leishmania infantum
S-adenosyl-L-homocysteine
-
 Leishmania infantum
S-adenosylmethionine more than 50% inhibition at 1 mM Leishmania infantum
tripolyphosphate competitive with ATP; non competitive with L-methionine Leishmania infantum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 native MAT-II, gel filtration Leishmania infantum
249000
-
 recombinant MAT-II, gel filtration Leishmania infantum

Organism

Organism UniProt Comment Textmining
Leishmania infantum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant MAT II purified by a method that includes Ni-agarose bead affinity capture. Alternative method includes Triton X-100 and 8 mM urea buffered extraction of inclusion body phase and dialysis Leishmania infantum

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
 Leishmania infantum phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Leishmania infantum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000883
-
 ATP
-
 Leishmania infantum
0.0000883
-
 S-adenosylmethionine
-
 Leishmania infantum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 tripolyphosphate inhibition with respect to L-methionine Leishmania infantum
0.25
-
 tripolyphosphate inhibition with respect to ATP Leishmania infantum
0.3
-
 diphosphate inhibition with respect to ATP Leishmania infantum
0.35
-
 diphosphate inhibition with respect to L-methionine Leishmania infantum
0.8
-
 ATP noncompetitive inhibition at 0.5-5 mM ATP concentrations Leishmania infantum
4
-
 S-adenosylmethionine noncompetitive inhibition at 0.5-5 mM L-methionine concentrations Leishmania infantum