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Literature summary for 2.5.1.6 extracted from

  • Markham, G.D.; Hafner, E.W.; White Tabor, C.; Tabor, H.
    S-Adenosylmethionine synthetase from Escherichia coli (1980), J. Biol. Chem., 255, 9082-9092.
    View publication on PubMed
Search for more literature for 2.5.1.6

Activating Compound

Activating Compound Comment Organism Structure
S-adenosylmethionine enhances tripolyphosphatase activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Adenyl-5'-ylimidodiphosphate competitive with ATP Escherichia coli
diphosphate
-
 Escherichia coli
GTP
-
 Escherichia coli
phosphate
-
 Escherichia coli
S-adenosylmethionine
-
 Escherichia coli
S-carbamylcysteine competitive with methionine Escherichia coli
tripolyphosphate competitive with ATP Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Escherichia coli
Cs+
-
 Escherichia coli
K+
-
 Escherichia coli
Li+
-
 Escherichia coli
Mg2+ cation concentration must be at least equal to ATP concentration for maximal activity Escherichia coli
Mn2+
-
 Escherichia coli
additional information divalent cations are required for tripolyphosphatase activity Escherichia coli
Na+
-
 Escherichia coli
NH4+
-
 Escherichia coli
Tl+
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 4 * 43000, SDS-PAGE Escherichia coli
180000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0022
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxy-ATP + L-methionine + H2O
-
 Escherichia coli ?
-
 ?
3'-deoxy-ATP + L-methionine + H2O
-
 Escherichia coli ?
-
 ?
ATP + L-methionine + H2O
-
 Escherichia coli S-adenosyl-L-methionine + phosphate + diphosphate
-
 ?
additional information ATP can be substituted by 3'-deoxy-ATP,8-bromo-ATP, formycin triphosphate, adenyl-5'yl imidodiphosphate Escherichia coli ?
-
 ?
tripolyphosphate + H2O tripolyphosphatase activity Escherichia coli diphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 43000, SDS-PAGE Escherichia coli