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Literature summary for 2.5.1.6 extracted from
- Multiple species of mammalian S-adenosylmethionine synthetase. Partial purification and characterization (1981), Biochemistry, 20, 934-940.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dimethylsulfoxide | activates alpha and beta isoenzymes from liver | Rattus norvegicus |  |
| dithiothreitol | required for alpha and beta isoenzymes activity, not required for gamma enzyme | Rattus norvegicus | |
| glycerol | activates rat liver isoenzymes alpha and beta | Rattus norvegicus | |
| tripolyphosphates | activation or inhibition, depending on isoenzyme, S-adenosylmethionine and tripolyphosphate concentration | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Dimethylsulfoxide | slight inhibition of gamma isoenzyme from kidney | Rattus norvegicus | |
| glycerol | inhibits kidney isoenzyme gamma | Rattus norvegicus | |
| p-chloromercuribenzoate | alpha and beta isoenzymes completely inhibited, gamma isoenzyme slightly inhibited | Rattus norvegicus | |
| tripolyphosphate | activation or inhibition, depending on isoenzyme, S-adenosylmethionine and tripolyphosphate concentration | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | absolute requirement, cannot be replaced by Na+ | Rattus norvegicus | |
| Mg2+ | absolute requirement, cannot be replaced by Mn2+ | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
beta isoenzyme from liver, gel filtration | Rattus norvegicus |
| 190000 | - |
gamma isoenzyme from kidney, gel filtration | Rattus norvegicus |
| 210000 | - |
alpha isoenzyme, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gamma isoenzyme from kidney | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | gamma isoenzyme in kidney enzyme | Rattus norvegicus | - |
| liver | alpha and beta isozymes | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3 | - |
beta isoenzyme from liver | Rattus norvegicus |
| 7.5 | - |
alpha isoenzyme from liver | Rattus norvegicus |
| 39 | - |
gamma isoenzyme from liver | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 50 mM Tris/HCl, pH 7.8, 20% v/v glycerol, 0.2 mM DTT, 0.1 mM EDTA, 10 mM MgCl2, 0.1 M KCl, partially purified liver enzymes stable for at least 1 month | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-ATP + L-methionine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3'-deoxy-ATP + L-methionine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| ATP + L-methionine + H2O | - |
Rattus norvegicus | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | about 70-80% of maximal activity at pH 7.5 and 9.5 | Rattus norvegicus |
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