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Literature summary for 2.5.1.6 extracted from

  • Cabrero, C.; Puerta, J.; Alemany, S.
    Purification and comparison of two forms of S-adenosyl-L-methionine synthetase from rat liver (1987), Eur. J. Biochem., 170, 299-304.
    View publication on PubMed
Search for more literature for 2.5.1.6

Activating Compound

Activating Compound Comment Organism Structure
S-adenosylmethionine below 0.3 mM activates low-MW isoenzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
diphosphate inhibits high-MW isoenzyme, no effect on low-MW enzyme Rattus norvegicus
S-adenosylmethionine above 0.3 mM inhibits both high-MW and low-MW isoenzymes Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, low-MW isoenzyme, SDS-PAGE Rattus norvegicus
48500
-
 4 * 48500, high-MW isoenzyme, SDS-PAGE Rattus norvegicus
110000
-
 low-MW isoenzyme, gel filtration Rattus norvegicus
210000
-
 high-MW isoenzyme, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 2 forms: high-MW form and low-MW form
-

Purification (Commentary)

Purification (Comment) Organism
2 forms: high-MW form and low-MW form Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.28
-
 low-MW isozyme Rattus norvegicus
4.61
-
 high-MW isozyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxy-ATP + L-methionine + H2O
-
 Rattus norvegicus ?
-
 ?
3'-deoxy-ATP + L-methionine + H2O
-
 Rattus norvegicus ?
-
 ?
ATP + L-methionine + H2O
-
 Rattus norvegicus S-adenosyl-L-methionine + phosphate + diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 47000, low-MW isoenzyme, SDS-PAGE Rattus norvegicus
tetramer 4 * 48500, high-MW isoenzyme, SDS-PAGE Rattus norvegicus