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Literature summary for 2.5.1.6 extracted from

  • Kotb, M.; Kredich, N.M.
    S-Adenosylmethionine synthetase from human lymphocytes. Purification and characterization (1985), J. Biol. Chem., 260, 3923-3930.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Homo sapiens
Dimethylsulfoxide
-
Homo sapiens
diphosphate
-
Homo sapiens
L-methionine
-
Homo sapiens
additional information overview of the regulatory properties, effect of L-methionine analogues and influence of L-methionine concentration on activating and inhibiting effects, effect of tripolyphosphate and p-hydroxymercuribenzoate Homo sapiens
p-chloromercuribenzoate
-
Homo sapiens
phosphate
-
Homo sapiens
S-adenosylmethionine non competitive with ATP at low methionine concentration Homo sapiens
tripolyphosphate competitive with ATP and non competitive with L-methionine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
ATP
-
Homo sapiens
3.3
-
L-methionine
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ in excess of that bound to ATP and EDTA is required for optimal activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
185000
-
equilibrium sedimentation studies Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine steady state ordered Bi Ter mechanism with ATP adding before L-methionine and S-adenosylmethionine being the first product released, random release of phosphate and diphosphate Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
lymphocyte from chronic leukemia cells Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens
12.2
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, less than 10% loss of activity after 3 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxy-ATP + L-methionine + H2O
-
Homo sapiens ?
-
?
3'-deoxy-ATP + L-methionine + H2O
-
Homo sapiens ?
-
?
ATP + L-methionine + H2O
-
Homo sapiens S-adenosyl-L-methionine + phosphate + diphosphate
-
?
tripolyphosphate + H2O tripolyphosphatase activity Homo sapiens diphosphate + phosphate
-
?

Subunits

Subunits Comment Organism
?
-
Homo sapiens
tetramer heterotetramer with subunit composition alpha-alpha'-beta2, alpha2beta2 or alpha'2beta2, where alpha, alpha', and beta are chains of molecular weight 53000, 51000 and 38000 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
stable for several hours, if protein concentration is above 0.1 mg/ml and ionic strength is at least 50 mM Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5
-
Homo sapiens