Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cycloleucine | - |
Trypanosoma brucei brucei | |
D-methionine | - |
Trypanosoma brucei brucei | |
Dimethylsulfoxide | - |
Trypanosoma brucei brucei | |
DL-Ethionine | - |
Trypanosoma brucei brucei | |
L-2-Amino-4-methoxy-cis-but-3-enoic acid | - |
Trypanosoma brucei brucei | |
Mg2+ | inhibitory above 8.5 mM | Trypanosoma brucei brucei | |
Mn2+ | inhibition in presence of Mg2+ | Trypanosoma brucei brucei | |
Na+ | in presence of Mg2+ | Trypanosoma brucei brucei | |
S-adenosyl-L-homocysteine | - |
Trypanosoma brucei brucei | |
S-adenosylmethionine | - |
Trypanosoma brucei brucei | |
seleno-L-methionine | - |
Trypanosoma brucei brucei | |
Sodium diphosphate | - |
Trypanosoma brucei brucei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement | Trypanosoma brucei brucei | |
Mn2+ | can partially replace Mg2+ in activation, inhibition in presence of Mg2+ | Trypanosoma brucei brucei | |
Na+ | slight activation in absence of Mg2+ | Trypanosoma brucei brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
145000 | - |
gel filtration | Trypanosoma brucei brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Trypanosoma brucei brucei | used as aminopropyl group donor in synthesis of polyamines and is also the methyl group donor for most cellular methyltransferase reactions | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Trypanosoma brucei brucei |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Trypanosoma brucei brucei |
0.00278 | - |
- |
Trypanosoma brucei brucei |
Storage Stability | Organism |
---|---|
-70°C, 63% loss of activity after 3 days | Trypanosoma brucei brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxy-ATP + L-methionine + H2O | - |
Trypanosoma brucei brucei | ? | - |
? | |
3'-deoxy-ATP + L-methionine + H2O | - |
Trypanosoma brucei brucei | ? | - |
? | |
ATP + L-ethionine + H2O | - |
Trypanosoma brucei brucei | S-adenosyl-L-ethionine + phosphate + diphosphate | - |
? | |
ATP + L-methionine + H2O | - |
Trypanosoma brucei brucei | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
ATP + L-methionine + H2O | used as aminopropyl group donor in synthesis of polyamines and is also the methyl group donor for most cellular methyltransferase reactions | Trypanosoma brucei brucei | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
additional information | ATP can be substituted by 3'-deoxy-ATP,8-bromo-ATP, formycin triphosphate, adenyl-5'yl imidodiphosphate | Trypanosoma brucei brucei | ? | - |
? | |
additional information | methionine can be substituted by selenomethionine, alpha-methyl-DL-methionine | Trypanosoma brucei brucei | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma brucei brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
slight variation in activity between 8 and 10 | Trypanosoma brucei brucei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
S-adenosylmethionine | - |
Trypanosoma brucei brucei | |
0.4 | - |
D-methionine | - |
Trypanosoma brucei brucei | |
0.51 | - |
seleno-L-methionine | - |
Trypanosoma brucei brucei | |
0.71 | - |
DL-Ethionine | - |
Trypanosoma brucei brucei | |
1.6 | - |
Sodium diphosphate | - |
Trypanosoma brucei brucei | |
17 | - |
cycloleucine | - |
Trypanosoma brucei brucei | |
250 | - |
Na+ | in presence of Mg2+ | Trypanosoma brucei brucei |