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Literature summary for 2.5.1.6 extracted from
- Fractionation and kinetic properties of rat liver and kidney methionine adenosyltransferase isozymes (1983), Biochemistry, 22, 1636-1641.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dimethylsulfoxide | activates isoform from kidney and isoform MAT-III from liver | Rattus norvegicus |  |
| S-adenosylmethionine | activation of rat liver MAT-III | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Dimethylsulfoxide | weak inhibition of liver isoenzyme | Rattus norvegicus | |
| S-adenosylmethionine | inhibition of rat kidney enzyme and rat liver MAT-II, weak inhibition of rat liver MAT-I | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
methionine | MAT-II isoenzyme | Rattus norvegicus | |
| 0.041 | - |
L-methionine | MAT-I isoenzyme | Rattus norvegicus | |
| 0.215 | - |
L-methionine | MAT-III isoenzyme | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
2 * 47000, MAT-III, SDS-PAGE | Rattus norvegicus |
| 97000 | - |
MAT-III, gel filtration | Rattus norvegicus |
| 120000 | - |
MAT-II, isolated from kidney and liver, gel filtration | Rattus norvegicus |
| 208000 | - |
MAT-I, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
3 isoenzymes: MAT-I, MAT-II, MAT-III | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| three isoforms: MAT-I, MAT-II and MAT-III, MAT-III to homogeneity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-ATP + L-methionine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3'-deoxy-ATP + L-methionine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| ATP + L-methionine + H2O | - |
Rattus norvegicus | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
| ATP + methionine + H2O | - |
Rattus norvegicus | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 47000, MAT-III, SDS-PAGE | Rattus norvegicus |
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