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Literature summary for 2.5.1.59 extracted from

  • Ashok, S.; Hildebrandt, E.R.; Ruiz, C.S.; Hardgrove, D.S.; Coreno, D.W.; Schmidt, W.K.; Hougland, J.L.
    Protein farnesyltransferase catalyzes unanticipated farnesylation and geranylgeranylation of shortened target sequences (2020), Biochemistry, 59, 1149-1162 .
    View publication on PubMed
Search for more literature for 2.5.1.59

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q04631 AND Q02293 Q04631 i.e. alpha-subunit, Q02293 i.e. beta-subunit, cf. EC 2.5.1.58
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + dansyl-GCFT
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 Rattus norvegicus diphosphate + S-geranylgeranyl dansyl-GCFT
-
 ?
geranylgeranyl diphosphate + dansyl-GCII
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 Rattus norvegicus diphosphate + S-geranylgeranyl dansyl-GCII
-
 ?
geranylgeranyl diphosphate + dansyl-GCLL
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 Rattus norvegicus diphosphate + S-geranylgeranyl dansyl-GCLL
-
 ?
geranylgeranyl diphosphate + dansyl-GCYL
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 Rattus norvegicus diphosphate + S-geranylgeranyl dansyl-GCYL
-
 ?
additional information enzyme prefers farnesyl diphosphate over geranylgeranyl diphosphate, but catalyzes the reactions of both substrates, i.e. reactions of EC 2.5.1.58 and EC 2.5.1.59, respectively Rattus norvegicus ?
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kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.7
-
 dansyl-GCII steady state reactivity, pH 7.8, 33°C Rattus norvegicus
0.92
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 dansyl-GCYL steady state reactivity, pH 7.8, 33°C Rattus norvegicus
1.7
-
 dansyl-GCFT steady state reactivity, pH 7.8, 33°C Rattus norvegicus
6.1
-
 dansyl-GCLL steady state reactivity, pH 7.8, 33°C Rattus norvegicus