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Literature summary for 2.5.1.58 extracted from

  • Hougland, J.L.; Gangopadhyay, S.A.; Fierke, C.A.
    Expansion of protein farnesyltransferase specificity using "tunable" active site interactions: development of bioengineered prenylation pathways (2012), J. Biol. Chem., 287, 38090-38100.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.58

Protein Variants

Protein Variants Comment Organism
W102F/W106E mutations in the beta-subunit. Mutations relax substrate selectivity without loss of activity Homo sapiens
W102L/W106E mutations in the beta-subunit. Mutations relax substrate selectivity without loss of activity Homo sapiens
W102L/W106R mutations in the beta-subunit. Mutations relax substrate selectivity without loss of activity Homo sapiens
W102R/W106K mutations in the beta-subunit. Mutations relax substrate selectivity without loss of activity Homo sapiens
W102R/W106L mutations in the beta-subunit. Mutations relax substrate selectivity without loss of activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 dansyl-GCVDS Km value clearly above 0.01 mM, wild-type, pH 7.8, 25°C Homo sapiens
additional information
-
 dansyl-GCVKS Km value clearly above 0.01 mM, wild-type, pH 7.8, 25°C Homo sapiens
0.0014
-
 dansyl-GCVKS mutant W102F/W106E, pH 7.8, 25°C Homo sapiens
0.003
-
 dansyl-GCVDS mutant W102L/W106R, pH 7.8, 25°C Homo sapiens
0.009
-
 dansyl-GCVDS mutant W102R/W106L, pH 7.8, 25°C Homo sapiens
0.01
-
 dansyl-GCVKS mutant W102L/W106E, pH 7.8, 25°C Homo sapiens
0.011
-
 dansyl-GCVDS mutant W102R/W106K, pH 7.8, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-GCVDS + (2E,6E)-farnesyl diphosphate poor substrate for wild-type Homo sapiens dansyl-G-(S-(2E,6E)-farnesyl)CVDS + diphosphate
-
 ?
dansyl-GCVKS + (2E,6E)-farnesyl diphosphate poor substrate for wild-type Homo sapiens dansyl-G-(S-(2E,6E)-farnesyl)CVKS + diphosphate
-
 ?