Application | Comment | Organism |
---|---|---|
medicine | - |
Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
wild-type enzyme and mutant enzymes expressed in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H248A | beta-subunit, mutation has no effect on substrate binding affinity, the farnesylation rate constant is 10fold decreased in comparison of wild-type, the rate constant by chemical step using farnesyl monophosphate 5fold decreased | Rattus norvegicus |
K164A | alpha-subunit, mutation has no effect on substrate binding affinity, the farnesylation rate constant is 30fold decreased in comparison of wild-type enzyme, the rate constant by chemical step using farnesyl monophosphate unaffected | Rattus norvegicus |
additional information | mutations have a little effect on the pH and magnesium dependence: side chains K164alpha, H248beta and Y300beta not function either as general acid-base catalyst or as magnesium ligands | Rattus norvegicus |
Y300F | beta-subunit, mutation has no effect on substrate binding affinity, the farnesylation rate constant is 500fold decreased in comparison of wild-type enzyme, the rate constant by chemical step using farnesyl monophosphate 300fold decreased. Transition state for farnesylation is stabilized by interactions between the alpha-phosphate of the isoprenoid substrate and the side chains of Y300beta | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc metalloenzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + protein-cysteine | prenylation, farnesylation, substrates are Ras, nuclear lamins, transducin gamma subunit, protein substrate motif: Cys-aliphatic-aliphatic-X, X: M, S, Q, A, F | Rattus norvegicus | diphosphate + S-farnesyl protein | - |
? | |
farnesyl monophosphate + Gly-Cys-Val-Leu-Ser | - |
Rattus norvegicus | phosphate + Gly-S-farnesyl-Cys-Val-Leu-Ser | - |
? |