Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
M42L | mutant shows the additional 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity like the wild type enzyme | Homo sapiens |
M42T | the mutation abolishes the 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity completely without compromising the Neu5Ac-9-P synthase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.04 | - |
N-acetyl-D-mannosamine 6-phosphate | wild type enzyme, at 37°C | Homo sapiens | |
1.26 | - |
N-acetyl-D-mannosamine 6-phosphate | mutant enzyme M42T, at 37°C | Homo sapiens | |
1.72 | - |
N-acetyl-D-mannosamine 6-phosphate | mutant enzyme M42L, at 37°C | Homo sapiens | |
2.5 | 5 | D-mannose 6-phosphate | mutant enzyme M42L, at 37°C | Homo sapiens | |
2.62 | - |
D-mannose 6-phosphate | wild type enzyme, at 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ chelating Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannose 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate + phosphate | - |
? | |
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | N-acetyl-neuraminate 9-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-acetylneuraminic acid 9-phosphate synthase | - |
Homo sapiens |
Neu5Ac 9-phosphate synthase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
D-mannose 6-phosphate | mutant enzyme M42L, at 37°C | Homo sapiens | |
0.011 | - |
D-mannose 6-phosphate | wild type enzyme, at 37°C | Homo sapiens | |
0.013 | - |
N-acetyl-D-mannosamine 6-phosphate | mutant enzyme M42T, at 37°C | Homo sapiens | |
0.016 | - |
N-acetyl-D-mannosamine 6-phosphate | mutant enzyme M42L, at 37°C | Homo sapiens | |
0.022 | - |
N-acetyl-D-mannosamine 6-phosphate | wild type enzyme, at 37°C | Homo sapiens |