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Literature summary for 2.5.1.57 extracted from

  • Hao, J.; Vann, W.F.; Hinderlich, S.; Sundaramoorthy, M.
    Elimination of 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity from human N-acetylneuraminic acid 9-phosphate synthase by a single mutation (2006), Biochem. J., 397, 195-201.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
M42L mutant shows the additional 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity like the wild type enzyme Homo sapiens
M42T the mutation abolishes the 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity completely without compromising the Neu5Ac-9-P synthase activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.04
-
N-acetyl-D-mannosamine 6-phosphate wild type enzyme, at 37°C Homo sapiens
1.26
-
N-acetyl-D-mannosamine 6-phosphate mutant enzyme M42T, at 37°C Homo sapiens
1.72
-
N-acetyl-D-mannosamine 6-phosphate mutant enzyme M42L, at 37°C Homo sapiens
2.5 5 D-mannose 6-phosphate mutant enzyme M42L, at 37°C Homo sapiens
2.62
-
D-mannose 6-phosphate wild type enzyme, at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ chelating Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannose 6-phosphate + phosphoenolpyruvate + H2O
-
Homo sapiens 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate + phosphate
-
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
Homo sapiens N-acetyl-neuraminate 9-phosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
N-acetylneuraminic acid 9-phosphate synthase
-
Homo sapiens
Neu5Ac 9-phosphate synthase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
D-mannose 6-phosphate mutant enzyme M42L, at 37°C Homo sapiens
0.011
-
D-mannose 6-phosphate wild type enzyme, at 37°C Homo sapiens
0.013
-
N-acetyl-D-mannosamine 6-phosphate mutant enzyme M42T, at 37°C Homo sapiens
0.016
-
N-acetyl-D-mannosamine 6-phosphate mutant enzyme M42L, at 37°C Homo sapiens
0.022
-
N-acetyl-D-mannosamine 6-phosphate wild type enzyme, at 37°C Homo sapiens