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Literature summary for 2.5.1.57 extracted from
- Cloning, expression, and characterization of sialic acid synthases (2005), Biochem. Biophys. Res. Commun., 338, 1507-1514.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1 mM, 93% decrease of activity | Homo sapiens |  |
| Ca2+ | 1 mM, 76% decrease of activity | Homo sapiens | |
| Co2+ | 1 mM, 15% decrease of activity | Homo sapiens | |
| Cu2+ | 1 mM, 68% decrease of activity | Homo sapiens | |
| EDTA | 10 mM, complete inhibition | Homo sapiens | |
| Fe2+ | 1 mM, 79% decrease of activity | Homo sapiens | |
| Ni2+ | 1 mM, 24% decrease of activity | Homo sapiens | |
| Zn2+ | 1 mM, 87% decrease of activity | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.04 | - |
N-acetyl-D-mannosamine 6-phosphate | in 50 mM bicine pH 7.5 at 37°C | Homo sapiens | |
| 2.62 | - |
D-mannose 6-phosphate | in 50 mM bicine pH 7.5 at 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 1 mM, 30% increase of activity | Homo sapiens | |
| Mn2+ | 1 mM, 15% increase of activity | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42360 | - |
calculated from sequence of cDNA | Homo sapiens |
| 42360 | - |
ESI mass spectrometry | Homo sapiens |
| 100100 | - |
SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-Sepharose Fast Flow column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannose 6-phosphate + ? | - |
Homo sapiens | ? | - |
? | |
| N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | N-acetyl-neuraminate 9-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer or trimer | the molecular weight is more than the size of a dimer, but smaller than a trimer | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N-acetylneuraminic acid-9-phosphate synthase | - |
Homo sapiens |
| NeuNAc-9-P synthase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 70 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.67 | - |
D-mannose 6-phosphate | in 50 mM bicine pH 7.5 at 37°C | Homo sapiens | |
| 1.31 | - |
N-acetyl-D-mannosamine 6-phosphate | in 50 mM bicine pH 7.5 at 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Homo sapiens |
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