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Literature summary for 2.5.1.57 extracted from
- The sialic acids. VIII. Sialic acid 9-phosphate synthetase (1966), J. Biol. Chem., 241, 5627-5636.
General Stability
| General Stability | Organism |
|---|---|
| purified enzyme is unstable to freezing and thawing | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Sus scrofa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.69 | - |
N-acetyl-D-mannosamine 6-phosphate | - |
Sus scrofa | |
| 1.6 | - |
N-glycolyl-D-mannosamine 6-phosphate | - |
Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Rattus norvegicus | |
| Fe2+ | - |
Sus scrofa | |
| Mg2+ | - |
Rattus norvegicus | |
| Mg2+ | essential, 0.01 M required for maximal activity | Sus scrofa | |
| Mn2+ | - |
Rattus norvegicus | |
| Mn2+ | - |
Sus scrofa | |
| additional information | Ca2+ or Cu2+ does not substitute for Mg2+ | Rattus norvegicus | |
| additional information | Ca2+ or Cu2+ does not substitute for Mg2+ | Sus scrofa | |
| additional information | Al3+ does not substitute for Mg2+ | Sus scrofa | |
| Ni2+ | - |
Rattus norvegicus | |
| Ni2+ | - |
Sus scrofa | |
| Zn2+ | - |
Sus scrofa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | Homo sapiens | - |
N-acetyl-neuraminate 9-phosphate + phosphate | - |
? | |
| N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | Rattus norvegicus | - |
N-acetyl-neuraminate 9-phosphate + phosphate | - |
? | |
| N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | Sus scrofa | - |
N-acetyl-neuraminate 9-phosphate + phosphate | - |
? | |
| N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | Ovis aries | - |
N-acetyl-neuraminate 9-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
| Ovis aries | - |
sheep | - |
| Rattus norvegicus | - |
rat | - |
| Sus scrofa | - |
hog | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Sus scrofa | - |
| blood | - |
Homo sapiens | - |
| brain | - |
Rattus norvegicus | - |
| brain | - |
Sus scrofa | - |
| erythrocyte | - |
Homo sapiens | - |
| erythrocyte | - |
Rattus norvegicus | - |
| heart | - |
Sus scrofa | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| muscle | - |
Rattus norvegicus | - |
| muscle | - |
Sus scrofa | - |
| ovary | - |
Sus scrofa | - |
| skin | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
| submaxillary gland | - |
Homo sapiens | - |
| submaxillary gland | - |
Sus scrofa | - |
| submaxillary gland | - |
Ovis aries | - |
| testis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.26 | - |
- |
Homo sapiens |
| 0.83 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, crude extract can be stored | Sus scrofa |
| 4°C, purified enzyme loses approximately half of its activity in 3 days | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | N-acetyl-neuraminate 9-phosphate + phosphate | - |
? | |
| N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Rattus norvegicus | N-acetyl-neuraminate 9-phosphate + phosphate | - |
? | |
| N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Sus scrofa | N-acetyl-neuraminate 9-phosphate + phosphate | - |
? | |
| N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Ovis aries | N-acetyl-neuraminate 9-phosphate + phosphate | - |
? | |
| N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Sus scrofa | N-acylneuraminate 9-phosphate + phosphate | - |
? | |
| N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | N-acylneuraminic acid 9-phosphate + phosphate | - |
? | |
| N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Sus scrofa | N-acylneuraminic acid 9-phosphate + phosphate | - |
? | |
| N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Ovis aries | N-acylneuraminic acid 9-phosphate + phosphate | - |
? | |
| N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | N-glycolyl-neuraminate 9-phosphate + phosphate | - |
? | |
| N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Sus scrofa | N-glycolyl-neuraminate 9-phosphate + phosphate | - |
? | |
| N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Ovis aries | N-glycolyl-neuraminate 9-phosphate + phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Sus scrofa |
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Release 2023.1 (January 2023)
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