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Literature summary for 2.5.1.56 extracted from

  • Garcia Garcia, M.I.; Lau, K.; von Itzstein, M.; Garcia Carmona, F.; Sanchez Ferrer, A.
    Molecular characterization of a new N-acetylneuraminate synthase (NeuB1) from Idiomarina loihiensis (2015), Glycobiology, 25, 115-123.
    View publication on PubMed
Search for more literature for 2.5.1.56

Cloned(Commentary)

Cloned (Comment) Organism
gene siaC, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta 2, subcloningin Escherichia coli strain DH5alpha Idiomarina loihiensis

Inhibitors

Inhibitors Comment Organism Structure
EDTA slightly inhibitory at 1 mM Idiomarina loihiensis
K+ 27% inhibition at 1 mM Idiomarina loihiensis
additional information no or poor effect by Mg2+ and Ca2+ at 1 mM Idiomarina loihiensis
Na+ slightly inhibitory at 1 mM Idiomarina loihiensis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slightly activating at 1 mM Idiomarina loihiensis
Mn2+ slightly activating at 1 mM Idiomarina loihiensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
-
 Idiomarina loihiensis
45000
-
-
 Idiomarina loihiensis
95000
-
 gel filtration Idiomarina loihiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O Idiomarina loihiensis
-
 phosphate + N-acetylneuraminate
-
 ir
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O Idiomarina loihiensis L2-TR
-
 phosphate + N-acetylneuraminate
-
 ir

Organism

Organism UniProt Comment Textmining
Idiomarina loihiensis Q5R082 gene siaC
-
Idiomarina loihiensis L2-TR Q5R082 gene siaC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2 by ultrafiltration, nickel affinity chromatography, and gel filtration Idiomarina loihiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows activity towards 2-acetamido-4-O-acetyl-2-deoxy-D-mannopyranose resulting in formation of 4-O-acetyl-ManNAc Idiomarina loihiensis ?
-
 ?
additional information the enzyme shows activity towards 2-acetamido-4-O-acetyl-2-deoxy-D-mannopyranose resulting in formation of 4-O-acetyl-ManNAc Idiomarina loihiensis L2-TR ?
-
 ?
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O
-
 Idiomarina loihiensis phosphate + N-acetylneuraminate
-
 ir
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O
-
 Idiomarina loihiensis L2-TR phosphate + N-acetylneuraminate
-
 ir

Subunits

Subunits Comment Organism
homodimer 2 * 45000, about, recombinant enzyme, SDS-PAGE, 2 * 41000, about, recombinant enzyme, HPLC/MS/ESI Idiomarina loihiensis
More the enzyme structure has a distinct domain architecture 4 without a cystathionine-beta-synthase domain (cystathionine-beta-synthetase domain), unlike domain architecture 3. The arginine essential for catalysis, that is present in antifreeze-like domain (block X), is not fully conserved in NeuB, but is replaced by a serine in domain architectures 3 and 4, three-dimensional structure comparison, and secondary structure element comparisons, detailed overview Idiomarina loihiensis

Synonyms

Synonyms Comment Organism
N-acetylneuraminate lyase synthase
-
 Idiomarina loihiensis
NeuB1
-
 Idiomarina loihiensis
sialic acid synthase UniProt Idiomarina loihiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Idiomarina loihiensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 purified recombinant enzyme, pH 8.0, reasonably stable, maintaining 60% activity after 4 h at 40, and 50% activity after 30 min at 50°C, and almost fully inactivated at 60°C after 1 h Idiomarina loihiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Idiomarina loihiensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 purified recombinant enzyme, 37°C, half-life is 8 h Idiomarina loihiensis
9
-
 purified recombinant enzyme, 37°C, half-life is 2 h, inactivation after 4 h Idiomarina loihiensis
10
-
 purified recombinant enzyme, 37°C, half-life is 1 h, inactivation after 3 h Idiomarina loihiensis

General Information

General Information Comment Organism
physiological function key enzyme in microorganisms for producing N-acetylneuraminic acid through the irreversible condensation of N-acetylmannosamine and phosphoenolpyruvate Idiomarina loihiensis