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Literature summary for 2.5.1.56 extracted from

  • Berg, T.O.; Gurung, M.K.; Altermark, B.; Smalas, A.O.; Raeder, I.L.
    Characterization of the N-acetylneuraminic acid synthase (NeuB) from the psychrophilic fish pathogen Moritella viscosa (2015), Carbohydr. Res., 402, 133-145.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme might be useful for production of sialic acids and derivates at low temperatures Moritella viscosa

Cloned(Commentary)

Cloned (Comment) Organism
gene MvNeuB, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) pLysS Moritella viscosa

Inhibitors

Inhibitors Comment Organism Structure
Co2+ stabilizes at low and inhibits at higher concentrations Moritella viscosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, overview Moritella viscosa
0.8
-
phosphoenolpyruvate pH 7.3, 30°C, recombinant enzyme Moritella viscosa
18
-
N-acetyl-D-mannosamine pH 7.3, 30°C, recombinant enzyme Moritella viscosa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates and stabilizes at low and inhibits at higher concentrations Moritella viscosa
Mg2+ requires Mn2+ or Mg2+, activates and stabilizes Moritella viscosa
Mn2+ requires Mn2+ or Mg2+, activates and stabilizes Moritella viscosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
-
Moritella viscosa
74000
-
recombinant enzyme, gel filtration Moritella viscosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O Moritella viscosa
-
phosphate + N-acetylneuraminate
-
?

Organism

Organism UniProt Comment Textmining
Moritella viscosa A0A090IMH4 gene MvNeuB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS by nickel affinity chromatography and gel filtration Moritella viscosa

Storage Stability

Storage Stability Organism
4°C, purified His6-tagged recombinant enzyme, stable for only 3-4 weeks, the enzyme readily aggregated at room temperature Moritella viscosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O
-
Moritella viscosa phosphate + N-acetylneuraminate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 38935, sequence calculation, 2 * 38000, about, recombinant enzyme, SDS-PAGE Moritella viscosa
More structure comparisons Moritella viscosa

Synonyms

Synonyms Comment Organism
N-acetylneuraminic acid synthase
-
Moritella viscosa
NeuB
-
Moritella viscosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
recombinant enzyme Moritella viscosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 50 the relative enzyme activity is approximately 20% at 5°C, and increasing to a maximum at around 30°C, and decreases rapidly above 30°C, no activity remains at 55°C. The enzyme from a psychrophilic bacterium, MvNeuB, is less stable at higher temperature and has a high catalytic activity at lower temperature compared to mesophilic counterparts. MvNeuB is a typical cold adapted enzyme Moritella viscosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the enzyme from a psychrophilic bacterium, MvNeuB, is less stable at higher temperature and has a high catalytic activity at lower temperature compared to mesophilic counterparts. MvNeuB is a typical cold adapted enzyme Moritella viscosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.72
-
N-acetyl-D-mannosamine pH 7.3, 30°C, recombinant enzyme Moritella viscosa
3.75
-
phosphoenolpyruvate pH 7.3, 30°C, recombinant enzyme Moritella viscosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
recombinant enzyme Moritella viscosa

pH Range

pH Minimum pH Maximum Comment Organism
7 10 activity range, inactive at pH 6.5, profile overview Moritella viscosa