Application | Comment | Organism |
---|---|---|
synthesis | the enzyme might be useful for production of sialic acids and derivates at low temperatures | Moritella viscosa |
Cloned (Comment) | Organism |
---|---|
gene MvNeuB, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) pLysS | Moritella viscosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stabilizes at low and inhibits at higher concentrations | Moritella viscosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Moritella viscosa | |
0.8 | - |
phosphoenolpyruvate | pH 7.3, 30°C, recombinant enzyme | Moritella viscosa | |
18 | - |
N-acetyl-D-mannosamine | pH 7.3, 30°C, recombinant enzyme | Moritella viscosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates and stabilizes at low and inhibits at higher concentrations | Moritella viscosa | |
Mg2+ | requires Mn2+ or Mg2+, activates and stabilizes | Moritella viscosa | |
Mn2+ | requires Mn2+ or Mg2+, activates and stabilizes | Moritella viscosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
- |
Moritella viscosa |
74000 | - |
recombinant enzyme, gel filtration | Moritella viscosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O | Moritella viscosa | - |
phosphate + N-acetylneuraminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moritella viscosa | A0A090IMH4 | gene MvNeuB | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS by nickel affinity chromatography and gel filtration | Moritella viscosa |
Storage Stability | Organism |
---|---|
4°C, purified His6-tagged recombinant enzyme, stable for only 3-4 weeks, the enzyme readily aggregated at room temperature | Moritella viscosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O | - |
Moritella viscosa | phosphate + N-acetylneuraminate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38935, sequence calculation, 2 * 38000, about, recombinant enzyme, SDS-PAGE | Moritella viscosa |
More | structure comparisons | Moritella viscosa |
Synonyms | Comment | Organism |
---|---|---|
N-acetylneuraminic acid synthase | - |
Moritella viscosa |
NeuB | - |
Moritella viscosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Moritella viscosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 50 | the relative enzyme activity is approximately 20% at 5°C, and increasing to a maximum at around 30°C, and decreases rapidly above 30°C, no activity remains at 55°C. The enzyme from a psychrophilic bacterium, MvNeuB, is less stable at higher temperature and has a high catalytic activity at lower temperature compared to mesophilic counterparts. MvNeuB is a typical cold adapted enzyme | Moritella viscosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme from a psychrophilic bacterium, MvNeuB, is less stable at higher temperature and has a high catalytic activity at lower temperature compared to mesophilic counterparts. MvNeuB is a typical cold adapted enzyme | Moritella viscosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.72 | - |
N-acetyl-D-mannosamine | pH 7.3, 30°C, recombinant enzyme | Moritella viscosa | |
3.75 | - |
phosphoenolpyruvate | pH 7.3, 30°C, recombinant enzyme | Moritella viscosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
recombinant enzyme | Moritella viscosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | activity range, inactive at pH 6.5, profile overview | Moritella viscosa |