Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli (BL21 deltaDE3) with pCWori+ vector | Neisseria meningitidis |
Crystallization (Comment) | Organism |
---|---|
purified and concentrated enzyme crystallized at 18°C, hanging-drop vapor diffusion, 10 mM MnCl2, 1.50-1.55 M malic acid, pH 6.2, soaked in 2 M sodium phosphate, pH 6.2, with 10 mM MnCl2, 25% ethylene glycol, frozen in liquid nitrogen at -173°C, complex with Mn2+ and 4.5/1 mixture of 2S-inhibitor/2R-inhibitor | Neisseria meningitidis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-acetamido-4,6,7,8,9-pentahydroxy-2-phosphorylnonanoic acid ditriethylammonium salt | 4.5/1 mixture of 2S-inhibitor/2R-inhibitor, competitive inhibitor against phosphoenolpyruvate | Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.157 | - |
phosphoenolpyruvate | 37°C, 100 mM Tris-HCl buffer, pH 7.0, 1 mM MnCl2, 50 microM to 1 mM phosphoenolpyruvate, His-tagged enzyme, purine nucleoside phosphorylase, 200 mircoM 2-amino-6-mercapto-7-methylpurine ribonucleoside | Neisseria meningitidis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O | Neisseria meningitidis | - |
N-acetylneuraminate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | E0NCD4 | - |
- |
Purification (Comment) | Organism |
---|---|
cell harvesting by centrifugation, resuspension, lysing in high-pressure homogenizer, centrifugation, chromatographic procedures including ion-exchange and gel filtration steps, concentration | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O | - |
Neisseria meningitidis | N-acetylneuraminate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NeuB | - |
Neisseria meningitidis |
sialic acid synthase | - |
Neisseria meningitidis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
5-acetamido-4,6,7,8,9-pentahydroxy-2-phosphorylnonanoic acid ditriethylammonium salt | 4.5/1 mixture of 2S-inhibitor/2R-inhibitor, a 1.2/1 mixture of 2S-inhibitor/2R-inhibitor requires a 2.5-fold lower total inhibitor concentration, 37°C, 100 mM Tris-HCl buffer, pH 7.0, 1 mM MnCl2, 50 microM to 1 mM phosphoenolpyruvate, His-tagged enzyme, purine nucleoside phosphorylase, 200 mircoM 2-amino-6-mercapto-7-methylpurine ribonucleoside | Neisseria meningitidis |
General Information | Comment | Organism |
---|---|---|
metabolism | formation of sialic acid or N-acetylneuraminic acid to produce a capsular polysacccharide of polysialic acid to evade the host's immune system by mimicking its own cell surface polysaccharides | Neisseria meningitidis |