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Literature summary for 2.5.1.56 extracted from

  • Sundaram, A.K.; Pitts, L.; Muhammad, K.; Wu, J.; Betenbaugh, M.; Woodard, R.W.; Vann, W.F.
    Characterization of N-acetylneuraminic acid synthase isoenzyme 1 from Campylobacter jejuni (2004), Biochem. J., 383, 83-89.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, complete inhibition Campylobacter jejuni
Zn2+ 0.1 mM, 68% of initial activity Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5
-
N-propanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
7.3
-
phosphoenolpyruvate pH 8.0, 37°C Campylobacter jejuni
15
-
N-butanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
17.6
-
N-acetyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
31.1
-
N-pentanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.1 mM, 122% of initial activity Campylobacter jejuni
Mg2+ 0.1 mM, 101% of initial activity Campylobacter jejuni
Mn2+ 0.1 mM, 150% of initial activity Campylobacter jejuni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43300
-
x * 43300, His-tagged recombinant enzyme, MALDI-TOF MS Campylobacter jejuni

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.018
-
pH 8.0, 37°C Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme catalyzes the condensation of the si face of phosphoenolpyruvate with the aldehyde sugar Campylobacter jejuni ?
-
?
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O
-
Campylobacter jejuni phosphate + N-acetylneuraminate
-
?
phosphoenolpyruvate + N-butanoyl-D-mannosamine + H2O
-
Campylobacter jejuni phosphate + N-butanoylneuraminate
-
?
phosphoenolpyruvate + N-pentanoyl-D-mannosamine + H2O
-
Campylobacter jejuni phosphate + N-pentanoylneuraminate
-
?
phosphoenolpyruvate + N-propanoyl-D-mannosamine + H2O
-
Campylobacter jejuni phosphate + N-propanoylneuraminate
-
?

Subunits

Subunits Comment Organism
? x * 43300, His-tagged recombinant enzyme, MALDI-TOF MS Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
N-pentanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
3.5
-
N-butanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
11.8
-
N-propanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
19
-
phosphoenolpyruvate pH 8.0, 37°C Campylobacter jejuni
19.9
-
N-acetyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni