Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Streptococcus agalactiae |
General Stability | Organism |
---|---|
expression of the native neuB gene product enzyme in E. coli results in a product that is prone to proteolysis during purification so the protein is tagged with a hexa-histidine tag at its N-terminus and rapidly purified | Streptococcus agalactiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 10 mM CuCl2, 90% inhibition | Streptococcus agalactiae | |
EDTA | 1 or 10 mM, 97% inhibition | Streptococcus agalactiae | |
Fe2+ | 1 mM FeCl2, 40% inhibition | Streptococcus agalactiae | |
K+ | 1 mM KCl, 92% inhibition | Streptococcus agalactiae | |
Li+ | 1 mM LiCl, 30% inhibition | Streptococcus agalactiae | |
Na+ | 1 mM NaCl, 20% inhibition | Streptococcus agalactiae | |
Phenylglyoxal | N-acetylmannosamine or phosphoenolpyruvate protect | Streptococcus agalactiae | |
Zn2+ | 1 mM ZnCl2, 80% inhibition | Streptococcus agalactiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM stimulates around 2fold, the enzyme is dependent on the presence of metal ions such as Mg2+, Mn2+ or Co2+ | Streptococcus agalactiae | |
Mg2+ | the enzyme is dependent on the presence of metal ions such as Mg2+, Mn2+ or Co2+ | Streptococcus agalactiae | |
Mn2+ | 1 mM stimulates around 2fold, the enzyme is dependent on the presence of metal ions such as Mg2+, Mn2+ or Co2+ | Streptococcus agalactiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38987 | - |
2 * 38987, electrospray ionisation mass spectrometry | Streptococcus agalactiae |
78000 | - |
gel filtration | Streptococcus agalactiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | Streptococcus agalactiae | the enzyme is involved in sialic acid synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus agalactiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
expression of the native neuB gene product enzyme in E. coli results in a product that is prone to proteolysis during purification so the protein is tagged with a hexa-histidine tag at its N-terminus and rapidly purified | Streptococcus agalactiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | arginine residues are present in the active site and are involved in substrate recognition and binding | Streptococcus agalactiae | ? | - |
? | |
N-acetyl-D-galactosamine + phosphoenolpyruvate + H2O | 15% of the activity with N-acetyl-D-mannosamine | Streptococcus agalactiae | ? | - |
? | |
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | - |
Streptococcus agalactiae | N-acetylneuraminate + phosphate | - |
? | |
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | the enzyme is involved in sialic acid synthesis | Streptococcus agalactiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38987, electrospray ionisation mass spectrometry | Streptococcus agalactiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
thermostable up to | Streptococcus agalactiae |
50 | - |
30 min, 80-90% loss of activity | Streptococcus agalactiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptococcus agalactiae |