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Literature summary for 2.5.1.56 extracted from

  • Komaki, E.; Ohta, Y.; Tsukada, Y.
    Purification and characterization of N-acetylneuraminate synthase from Escherichia coli K1-M12 (1997), Biosci. Biotechnol. Biochem., 61, 2046-2050.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 1 mM, 61% inhibition Escherichia coli
Cu2+ 1 mM CuCl2, 29% inhibition Escherichia coli
HgCl2 at 0.01 mM 85% inhibition, at 0.1 mM complete inhibition Escherichia coli
hydroxylamine 1 mM, 23% inhibition Escherichia coli
Iodine 1 mM, 30% inhibition Escherichia coli
N-bromosuccinimide 1 mM, 24% inhibition Escherichia coli
PCMB 1 mM, complete inhibition Escherichia coli
Semicarbazide 1 mM, 20% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
phosphoenolpyruvate pH 8.2, 37°C Escherichia coli
0.042
-
phosphoenolpyruvate pH 8.2, 37°C Escherichia coli
5.6
-
N-acetyl-D-mannosamine pH 8.2, 37°C Escherichia coli
6.25
-
N-acetyl-D-mannosamine pH 8.2, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion requirement Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
2 * 52000, SDS-PAGE Escherichia coli
106000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli K1-M12
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
487
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O
-
Escherichia coli N-acetylneuraminate + phosphate
-
?
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O
-
Escherichia coli K1-M12 N-acetylneuraminate + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 52000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 about 60% of maximal activity at 20°C and at 60°C Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 7.5, 30 min, stable up to Escherichia coli
40
-
pH 7.5, 30 min, about 60% loss of activity Escherichia coli
50
-
pH 7.5, 30 min, about 80% loss of activity Escherichia coli
60
-
pH 7.5, 30 min, complete loss of activity Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
4 12 pH 4.0: about 50% of maximal activity, pH 12.0: about 40% of maximal activity Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
25°C, 1 h, about 50% loss of activity Escherichia coli
5
-
25°C, 1 h, about 40% loss of activity Escherichia coli
6
-
25°C, 1 h, about 25% loss of activity Escherichia coli
7 10 25°C, 1 h, stable Escherichia coli
11
-
25°C, 1 h, about 40% loss of activity Escherichia coli
12
-
25°C, 1 h, about 60% loss of activity Escherichia coli