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Literature summary for 2.5.1.56 extracted from

  • Blacklow, R.S.; Warren, L.
    Biosynthesis of sialic acids by Neisseria meningitidis (1962), J. Biol. Chem., 237, 3520-3526.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glutathione required for maximal activity Neisseria meningitidis

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, complete inhibition Neisseria meningitidis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
phosphoenolpyruvate
-
Neisseria meningitidis
6.25
-
N-acetyl-D-mannosamine
-
Neisseria meningitidis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ at optimal concentration 50% of the activation with Mg2+ Neisseria meningitidis
Mg2+ at optimal concentration 50% of the activation with Mg2+ Neisseria meningitidis
Mn2+ divalent metal required Neisseria meningitidis
Mn2+ Mn2+ is most effective, optimal concentration: 5 mM Neisseria meningitidis

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Neisseria meningitidis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
-
Neisseria meningitidis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O specific for Neisseria meningitidis N-acetylneuraminate + phosphate
-
ir
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O the reaction strongly favors the formation of N-acetylneuraminic acid and appears to be irreversible Neisseria meningitidis N-acetylneuraminate + phosphate
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
in Tris buffer Neisseria meningitidis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 pH 6.5: about 40% of maximal activity, pH 10.0: about 60% of maximal activity Neisseria meningitidis