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Literature summary for 2.5.1.55 extracted from
- Campylobacter jejuni KDO8P Synthase, its inhibition by KDO8P oxime, and control of the residence time of slow-binding inhibition (2018), Biochemistry, 57, 5327-5338 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Campylobacter jejuni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-deoxy-D-manno-octulosonate 8-phosphate oxime | uncompetitive-like inhibition with respect to Mn2+ and competitive with respect to substrates, both fast-binding inhibition (Ki value 10 microM) and slow-binding inhibition (Ki* value 0.57 microM) | Campylobacter jejuni |  |
| EDTA | reversible inactivation | Campylobacter jejuni | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
D-arabinose 5-phosphate | 37°C, pH 7.5 | Campylobacter jejuni | |
| 0.65 | - |
phosphoenolpyruvate | 37°C, pH 7.5 | Campylobacter jejuni | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | full reactivation of EDTA-treated enzyme | Campylobacter jejuni | |
| Mn2+ | full reactivation of EDTA-treated enzyme, Km value 0.130 mM | Campylobacter jejuni | |
| additional information | reactivation of EDTA-treated enzyme, in decreasing order: Mn2+ = Co2+ = Ni2+ = Fe2+ > Ca2+ = Mg2+ = Cd2+ > Zn2+ > Cu2+ | Campylobacter jejuni | |
| Ni2+ | full reactivation of EDTA-treated enzyme | Campylobacter jejuni | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 123000 | - |
gel filtration | Campylobacter jejuni |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Campylobacter jejuni | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the kinetic mechanism is sequential ordered ter ter, with Mn2+ binding first | Campylobacter jejuni | ? | - |
- |
|
| phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | - |
Campylobacter jejuni | 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 29300, calulated from sequence | Campylobacter jejuni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Kdo8P synthase | - |
Campylobacter jejuni |
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Release 2023.1 (January 2023)
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