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Literature summary for 2.5.1.55 extracted from
- Conservation of the 2-keto-3-deoxymanno-octulosonic acid (Kdo) biosynthesis pathway between plants and bacteria (2013), Carbohydr. Res., 380, 70-75.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene kdsA1, in Arabidopsis a functionally redundant gene pair encodes Kdo-8-P synthases, AtKdsA1 (At1g9500) and AtKdsA2 (At1g16340), recombinant expression in Escherichia coli | Arabidopsis thaliana |
| gene kdsA2, in Arabidopsis a functionally redundant gene pair encodes Kdo-8-P synthases, AtKdsA1 (At1g9500) and AtKdsA2 (At1g16340), recombinant expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | gene kdsA1 single knockout and kdsA1/kdsA2 double knockout mutant constructions | Arabidopsis thaliana |
| additional information | gene kdsA2 single knockout and kdsA1/kdsA2 double knockout mutant constructions | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6H-6-Imino-(2,3,4,5-tetrahydropyrimido)[1,2-c]-[1,3]benzothiazine | PD404,182,displays strong inhibition in vitro, but is ineffective in vivo against Gram-negative bacteria due to an inability to cross the bacterial plasma membran | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | Escherichia coli | - |
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | Arabidopsis thaliana | - |
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q6NQL4 | gene kdsA2; gene kdsA2 | - |
| Arabidopsis thaliana | Q9AV97 | gene kdsA1; gene kdsA1 | - |
| Escherichia coli | - |
gene kdsA | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | the catalytic mechanism is thought to occur by correct substrate proximity and orientation through a series of sequential conformational changes in which phosphoenolpyruvate binding precedes that of D-arabinose 5-phosphate and the release of inorganic phosphate (Pi) precedes dissociation of the 3-deoxy-D-manno-octulosonate 8-phosphate product | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | - |
Escherichia coli | 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | - |
Arabidopsis thaliana | 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | in solution | Arabidopsis thaliana |
| tetramer | tetrameric quaternary structure with an active site in each monomer, by NMR study and crystal structure analysis | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KDO-8-P synthase | - |
Escherichia coli |
| KDO-8-P synthase | - |
Arabidopsis thaliana |
| Kdo-8-phosphate synthase | - |
Escherichia coli |
| Kdo-8-phosphate synthase | - |
Arabidopsis thaliana |
| KDOPS | - |
Escherichia coli |
| KDOPS | - |
Arabidopsis thaliana |
| KdsA | - |
Arabidopsis thaliana |
| KdsA | - |
Escherichia coli |
| KdsA1 | - |
Arabidopsis thaliana |
| KdsA2 | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.9 | - |
phosphoenolpyruvate | pH and temperature not specified in the publication, recombinant enzyme | Arabidopsis thaliana | |
| 5.9 | - |
D-arabinose 5-phosphate | pH and temperature not specified in the publication, recombinant enzyme | Arabidopsis thaliana | |
| 6.8 | - |
phosphoenolpyruvate | pH and temperature not specified in the publication | Escherichia coli | |
| 6.8 | - |
D-arabinose 5-phosphate | pH and temperature not specified in the publication | Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000026 | - |
6H-6-Imino-(2,3,4,5-tetrahydropyrimido)[1,2-c]-[1,3]benzothiazine | pH and temperature not specified in the publication | Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | Arabidopsis thaliana kdsA male gametophyte with lethal phenotype | Arabidopsis thaliana |
| malfunction | while single T-DNA mutants of either gene display no visible phenotypes, generation of a double knockout mutant has not been possible due to a failure of haploid pollen tube elongation | Arabidopsis thaliana |
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