Cloned (Comment) | Organism |
---|---|
1 15.5-kb segment containing the kdsA gene, expression in Escherichia coli | Aquifex pyrophilus |
Protein Variants | Comment | Organism |
---|---|---|
C11A | mutant enzyme retains less than 1% of the wild-type activity and is incapable of metal binding. Activity is not stimulated by Mn2+, Co2+ and Zn2+. Cd2+ stimulates 2fold at a concentration above 1 mM | Aquifex pyrophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Aquifex pyrophilus | |
EDTA | - |
Aquifex pyrophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
D-arabinose 5-phosphate | 60°C, pH 7.0, 0.48 mM Cd2+ | Aquifex pyrophilus | |
0.29 | - |
phosphoenolpyruvate | 60°C, pH 7.0, 0.48 mM Cd2+ | Aquifex pyrophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | stimulates sild-type enzyme above 0.4 mM, stimulation of mutant enzyme C11A above 1 mM | Aquifex pyrophilus | |
Co2+ | stimulates wild-type enzyme above 0.4 mM, no stimulation of mutant enzyme C11A | Aquifex pyrophilus | |
Iron | enzyme contains 0.19 mol of iron per mol of subunit | Aquifex pyrophilus | |
Mn2+ | stimulates wild-type enzyme above 0.4 mM, no stimulation of mutant enzyme C11A | Aquifex pyrophilus | |
Zinc | enzyme contains 0.26 mol of zinc per mol of subunit | Aquifex pyrophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex pyrophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Aquifex pyrophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Aquifex pyrophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Aquifex pyrophilus | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase | - |
Aquifex pyrophilus |
Kdo8P synthase | - |
Aquifex pyrophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
in 10-min reaction assay | Aquifex pyrophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | 60°C: about 30% of maximal activity, 70°C: about 70% of maximal activity, 90°C: about 40% of maximal activity | Aquifex pyrophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life: 8.0 h | Aquifex pyrophilus |
80 | - |
half-life: 2.25 h | Aquifex pyrophilus |
90 | - |
half-life: 0.5 h | Aquifex pyrophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
phosphoenolpyruvate | 60°C, pH 7.0, 0.48 mM Cd2+ | Aquifex pyrophilus | |
4 | - |
D-arabinose 5-phosphate | 60°C, pH 7.0, 0.48 mM Cd2+ | Aquifex pyrophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | in 10-min reaction assay | Aquifex pyrophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.5 | pH 4.5: about 50% of maximal activity, pH 7.5: about 50% of maximal activity | Aquifex pyrophilus |