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Literature summary for 2.5.1.55 extracted from

  • Shulami, S.; Yaniv, O.; Rabkin, E.; Shoham, Y.; Baasov, T.
    Cloning, expression, and biochemical characterization of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthase from the hyperthermophilic bacterium Aquifex pyrophilus (2003), Extremophiles, 7, 471-481.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
1 15.5-kb segment containing the kdsA gene, expression in Escherichia coli Aquifex pyrophilus

Protein Variants

Protein Variants Comment Organism
C11A mutant enzyme retains less than 1% of the wild-type activity and is incapable of metal binding. Activity is not stimulated by Mn2+, Co2+ and Zn2+. Cd2+ stimulates 2fold at a concentration above 1 mM Aquifex pyrophilus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Aquifex pyrophilus
EDTA
-
Aquifex pyrophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
D-arabinose 5-phosphate 60°C, pH 7.0, 0.48 mM Cd2+ Aquifex pyrophilus
0.29
-
phosphoenolpyruvate 60°C, pH 7.0, 0.48 mM Cd2+ Aquifex pyrophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ stimulates sild-type enzyme above 0.4 mM, stimulation of mutant enzyme C11A above 1 mM Aquifex pyrophilus
Co2+ stimulates wild-type enzyme above 0.4 mM, no stimulation of mutant enzyme C11A Aquifex pyrophilus
Iron enzyme contains 0.19 mol of iron per mol of subunit Aquifex pyrophilus
Mn2+ stimulates wild-type enzyme above 0.4 mM, no stimulation of mutant enzyme C11A Aquifex pyrophilus
Zinc enzyme contains 0.26 mol of zinc per mol of subunit Aquifex pyrophilus

Organism

Organism UniProt Comment Textmining
Aquifex pyrophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aquifex pyrophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2
-
-
Aquifex pyrophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-arabinose 5-phosphate
-
Aquifex pyrophilus 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase
-
Aquifex pyrophilus
Kdo8P synthase
-
Aquifex pyrophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
in 10-min reaction assay Aquifex pyrophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 60°C: about 30% of maximal activity, 70°C: about 70% of maximal activity, 90°C: about 40% of maximal activity Aquifex pyrophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
half-life: 8.0 h Aquifex pyrophilus
80
-
half-life: 2.25 h Aquifex pyrophilus
90
-
half-life: 0.5 h Aquifex pyrophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
phosphoenolpyruvate 60°C, pH 7.0, 0.48 mM Cd2+ Aquifex pyrophilus
4
-
D-arabinose 5-phosphate 60°C, pH 7.0, 0.48 mM Cd2+ Aquifex pyrophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 in 10-min reaction assay Aquifex pyrophilus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5 pH 4.5: about 50% of maximal activity, pH 7.5: about 50% of maximal activity Aquifex pyrophilus