Cloned (Comment) | Organism |
---|---|
cloned into a T7-driven expression vector, overexpression in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
phosphoenolpyruvate | 37°C | Arabidopsis thaliana | |
0.0038 | - |
D-arabinose 5-phosphate | 37°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | non-metallo enzyme, no metal requirement | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31519 | - |
2 * 31519, MALDI-MS | Arabidopsis thaliana |
76000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Arabidopsis thaliana | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31519, MALDI-MS | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-D-manno-octulosonate 8-phosphate synthase | - |
Arabidopsis thaliana |
KDOPS | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 70 | 45°C: about 50% of maximal activity, 70°C: about 55% of maximal activity | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, in absence of any substrate or in presence of D-arabinose 5-phosphate, 30% loss of activity, no inactivation in presence of phosphoenolpyruvate | Arabidopsis thaliana |
50 | - |
30 min, in absence of any substrate or in presence of D-arabinose 5-phosphate, 30% loss of activity, no inactivation in presence of phosphoenolpyruvate | Arabidopsis thaliana |
60 | - |
20% incrase in activity after 7 min | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
phosphoenolpyruvate | 37°C | Arabidopsis thaliana | |
5.9 | - |
D-arabinose 5-phosphate | 37°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | broad peak between pH 6.5 and 9.5 showing 90% of maximal activity | Arabidopsis thaliana |