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Literature summary for 2.5.1.54 extracted from
- Thermotoga maritima 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase: the ancestral eubacterial DAHP synthase? (2003), J. Biol. Chem., 278, 27525-27531.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | inactivation, reactivation in decreasing order by Zn2+, Cd2+, Mn2+, Cu2+, Co2+, Ni2+ | Thermotoga maritima |  |
| L-phenylalanine | 1 mM, 32% residual activity | Thermotoga maritima | |
| L-tyrosine | 1 mM, 23% residual activity | Thermotoga maritima | |
| additional information | not inhibitory: L-tryptophan, chorismate, shikimate, D-phenylalanine, L-histidine | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0095 | - |
phosphoenolpyruvate | pH 7.5, 70°C | Thermotoga maritima | |
| 0.0117 | - |
phosphoenolpyruvate | pH 7.5, 60°C | Thermotoga maritima | |
| 0.013 | - |
phosphoenolpyruvate | pH 7.5, 50°C | Thermotoga maritima | |
| 0.0573 | - |
D-erythrose 4-phosphate | pH 7.5, 50°C | Thermotoga maritima | |
| 0.138 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C | Thermotoga maritima | |
| 0.3506 | - |
D-erythrose 4-phosphate | pH 7.5, 70°C | Thermotoga maritima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | reactivation of EDTA-inactivated enzyme in decreasing order by Zn2+, Cd2+, Mn2+, Cu2+, Co2+, Ni2+ | Thermotoga maritima | |
| Zn2+ | - |
Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
4 * 38000, SDS-PAGE | Thermotoga maritima |
| 134000 | - |
gel filtration | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
recombinant enzyme | - |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reactivation of EDTA-inactivated enzyme in decreasing order by Zn2+, Cd2+, Mn2+, Cu2+, Co2+, Ni2+ | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Thermotoga maritima | 3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 38000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
86h, 50% residual activity | Thermotoga maritima |
| 70 | - |
21h, 50% residual activity | Thermotoga maritima |
| 80 | - |
5h, 50% residual activity | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
phosphoenolpyruvate | pH 7.5, 50°C | Thermotoga maritima | |
| 5 | - |
phosphoenolpyruvate | pH 7.5, 60°C | Thermotoga maritima | |
| 7.6 | - |
phosphoenolpyruvate | pH 7.5, 70°C | Thermotoga maritima | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
60°C | Thermotoga maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | more than 90% of maximum activity | Thermotoga maritima |
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Release 2023.1 (January 2023)
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