General Stability | Organism |
---|---|
overnight dialysis against buffer containing 10 mM EDTA at 4°C results in 85% inactivation | Streptomyces coelicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Neurospora crassa | |
EDTA | - |
Streptomyces coelicolor | |
Trp | Trp-sensitive isozyme | Neurospora crassa | |
Trp | Trp-sensitive isozyme | Streptomyces coelicolor | |
Trp | Trp-sensitive isozyme | Streptomyces rimosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.092 | - |
phosphoenolpyruvate | Trp-sensitive isozyme, pH 7.0, 30°C | Streptomyces coelicolor | |
0.195 | - |
D-erythrose 4-phosphate | Trp-sensitive isozyme, pH 7.0, 30°C | Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Neurospora crassa |
54000 | - |
2 * 54000, SDS-PAGE | Streptomyces coelicolor |
54000 | - |
2 * 54000, SDS-PAGE | Streptomyces rimosus |
107000 | - |
Trp-sensitive isozyme, gel filtration | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
tryptophan-sensitive isozyme | - |
Streptomyces coelicolor | - |
- |
- |
Streptomyces coelicolor | - |
Trp-sensitive isozyme | - |
Streptomyces coelicolor A3(2) | - |
- |
- |
Streptomyces rimosus | - |
Trp-sensitive isozyme | - |
Purification (Comment) | Organism |
---|---|
Trp-sensitive isozyme to homogeneity, 2000fold | Neurospora crassa |
Trp-sensitive isozyme to homogeneity, 2380fold, large scale | Streptomyces coelicolor |
Trp-sensitive isozyme to homogeneity, 3750fold | Streptomyces rimosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.7 | - |
purified Trp-sensitive isozyme | Neurospora crassa |
8.33 | - |
purified Trp-sensitive isozyme | Streptomyces coelicolor |
11.02 | - |
purified Trp-sensitive isozyme | Streptomyces rimosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Neurospora crassa | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Streptomyces coelicolor | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Streptomyces rimosus | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Streptomyces coelicolor A3(2) | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, SDS-PAGE | Neurospora crassa |
dimer | 2 * 54000, SDS-PAGE | Streptomyces coelicolor |
dimer | 2 * 54000, SDS-PAGE | Streptomyces rimosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces coelicolor |
7 | - |
assay at | Streptomyces rimosus |
7 | 7.4 | Trp-sensitive isozyme | Streptomyces coelicolor |