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Literature summary for 2.5.1.53 extracted from

  • Ahmmad, M.A.S.; Maskall, C.S.; Brown, E.G.
    Partial purification and properties of willardiine and isowillardiine synthase activity from Pisum sativum (1984), Phytochemistry, 23, 265-270.
No PubMed abstract available
Search for more literature for 2.5.1.53

Inhibitors

Inhibitors Comment Organism Structure
(2,4-Dihydroxypyrimidin-1-yl)-L-alanine i.e. willardiine or 3-(uracil-1-yl)-L-alanine, 30 mM, 84% inhibition of willardiine synthesis and 96% inhibition of isowillardiine synthesis Pisum sativum
(2,4-Dihydroxypyrimidin-3-yl)-L-alanine i.e. isowillardiine or 3-(uracil-3-yl)-L-alanine, 30 mM produces 75% inhibition of willardiine synthesis and 93% inhibition of synthesis of isowillardiine Pisum sativum
PCMB 0.001 mM, about 90% inhibition Pisum sativum
pyridoxal 5'-phosphate above 0.1 mM Pisum sativum
Tris-HCl buffer
-
 Pisum sativum
Uracil substrate inhibition above 35 mM Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
 Uracil synthesis of isowillardiine Pisum sativum
10
-
 Uracil willardiine synthesis Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 O3-acetyl-L-serine + 2 uracil Pisum sativum involved in biosynthesis of heterocyclic beta-substituted alanines 3-(uracil-1-yl)-L-alanine + 3-(uracil-3-yl)-L-alanine + 2 acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 pea, cv. Meteor
-

Purification (Commentary)

Purification (Comment) Organism
partial Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pisum sativum

Storage Stability

Storage Stability Organism
4°C, partially purified enzyme preparation, 30% loss of activity within 24 h, complete inactivation after 48 h Pisum sativum
liquid N2, crude enzyme preparation, in 3 M ammonium sulfate, 30% loss of activity within 8 weeks, beyond 8 weeks rapid decline of activity Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 O3-acetyl-L-serine + 2 uracil highly specific Pisum sativum 3-(uracil-1-yl)-L-alanine + 3-(uracil-3-yl)-L-alanine + 2 acetate
-
 ?
2 O3-acetyl-L-serine + 2 uracil involved in biosynthesis of heterocyclic beta-substituted alanines Pisum sativum 3-(uracil-1-yl)-L-alanine + 3-(uracil-3-yl)-L-alanine + 2 acetate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pisum sativum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 2 min, slow decrease of activity Pisum sativum
50 60 2 min, rapid decrease of activity Pisum sativum
70
-
 2 min, all activity ceased Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 7.9 O-acetylserine undergoes rapid O-acetyl-/N-acetyl shift above pH 8 Pisum sativum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pisum sativum