BRENDA - Enzyme Database
show all sequences of 2.5.1.52

Purification and characterization of L-mimosine synthase from Leucaema leucocephala

Murakoshi, I.; Ikegami, F.; Hinuma, Y.; Hanma, Y.; Phytochemistry 23, 1905-1908 (1984)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5
-
3,4-dihydroxypyridine
-
Leucaena leucocephala
6.25
-
O-acetylserine
-
Leucaena leucocephala
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, SDS-PAGE
Leucaena leucocephala
64000
-
gel filtration
Leucaena leucocephala
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O3-acetyl-L-serine + 3,4-dihydroxypyridine
Leucaena leucocephala
involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leucaena leucocephala
-
-
-
Purification (Commentary)
Commentary
Organism
partial, to near homogeneity
Leucaena leucocephala
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
cotyledons removed
Leucaena leucocephala
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.189
-
-
Leucaena leucocephala
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with O-phospho-L-serine and L-Ser
638434
Leucaena leucocephala
?
-
-
-
-
O-succinyl-L-serine + 3,4-dihydroxypyridine
60% of the activity with O-acetylserine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + succinate
-
-
-
?
O-sulfo-L-serine + 3,4-dihydroxypyridine
32% of the activity with O-acetyl-L-serine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + sulfate
-
-
-
?
O3-acetyl-L-serine + 3,4-dihydroxypyridine
best substrates
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
i.e. L-mimosine
638434
Leucaena leucocephala
?
O3-acetyl-L-serine + 3,4-dihydroxypyridine
involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
-
-
-
-
O3-acetyl-L-serine + 3-amino-1,2,4-triazole
30% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
-
638434
Leucaena leucocephala
?
O3-acetyl-L-serine + N-hydroxyurea
13% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
ureidoserine + acetate
-
638434
Leucaena leucocephala
?
O3-acetyl-L-serine + pyrazole
82% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
3-(pyrazol-1-yl)-L-alanine + acetate
-
638434
Leucaena leucocephala
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 32000, SDS-PAGE
Leucaena leucocephala
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
reaction with 3,4-dihydroxypyridine
Leucaena leucocephala
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit
Leucaena leucocephala
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit
Leucaena leucocephala
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5
-
3,4-dihydroxypyridine
-
Leucaena leucocephala
6.25
-
O-acetylserine
-
Leucaena leucocephala
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, SDS-PAGE
Leucaena leucocephala
64000
-
gel filtration
Leucaena leucocephala
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O3-acetyl-L-serine + 3,4-dihydroxypyridine
Leucaena leucocephala
involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial, to near homogeneity
Leucaena leucocephala
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
cotyledons removed
Leucaena leucocephala
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.189
-
-
Leucaena leucocephala
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with O-phospho-L-serine and L-Ser
638434
Leucaena leucocephala
?
-
-
-
-
O-succinyl-L-serine + 3,4-dihydroxypyridine
60% of the activity with O-acetylserine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + succinate
-
-
-
?
O-sulfo-L-serine + 3,4-dihydroxypyridine
32% of the activity with O-acetyl-L-serine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + sulfate
-
-
-
?
O3-acetyl-L-serine + 3,4-dihydroxypyridine
best substrates
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
i.e. L-mimosine
638434
Leucaena leucocephala
?
O3-acetyl-L-serine + 3,4-dihydroxypyridine
involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
-
-
-
-
O3-acetyl-L-serine + 3-amino-1,2,4-triazole
30% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
-
638434
Leucaena leucocephala
?
O3-acetyl-L-serine + N-hydroxyurea
13% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
ureidoserine + acetate
-
638434
Leucaena leucocephala
?
O3-acetyl-L-serine + pyrazole
82% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
3-(pyrazol-1-yl)-L-alanine + acetate
-
638434
Leucaena leucocephala
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 32000, SDS-PAGE
Leucaena leucocephala
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
reaction with 3,4-dihydroxypyridine
Leucaena leucocephala
Other publictions for EC 2.5.1.52
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
638434
Murakoshi
-
Purification and characterizat ...
Leucaena leucocephala
Phytochemistry
23
1905-1908
1984
-
-
-
-
-
-
-
2
-
-
2
1
-
1
-
-
1
-
-
1
1
-
8
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
1
1
-
8
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
638432
Murakoshi
-
The enzymic synthesis of beta- ...
Leucaena leucocephala
Phytochemistry
11
177-182
1972
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-