KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
3,4-dihydroxypyridine | - |
Leucaena leucocephala | |
6.25 | - |
O-acetylserine | - |
Leucaena leucocephala |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Leucaena leucocephala |
64000 | - |
gel filtration | Leucaena leucocephala |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O3-acetyl-L-serine + 3,4-dihydroxypyridine | Leucaena leucocephala | involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine | 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucaena leucocephala | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, to near homogeneity | Leucaena leucocephala |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | cotyledons removed | Leucaena leucocephala | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.189 | - |
- |
Leucaena leucocephala |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with O-phospho-L-serine and L-Ser | Leucaena leucocephala | ? | - |
? | |
O-succinyl-L-serine + 3,4-dihydroxypyridine | 60% of the activity with O-acetylserine | Leucaena leucocephala | 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + succinate | - |
? | |
O-sulfo-L-serine + 3,4-dihydroxypyridine | 32% of the activity with O-acetyl-L-serine | Leucaena leucocephala | 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + sulfate | - |
? | |
O3-acetyl-L-serine + 3,4-dihydroxypyridine | best substrates | Leucaena leucocephala | 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate | i.e. L-mimosine | ? | |
O3-acetyl-L-serine + 3,4-dihydroxypyridine | involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine | Leucaena leucocephala | 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate | - |
? | |
O3-acetyl-L-serine + 3-amino-1,2,4-triazole | 30% of the activity with 3,4-dihydroxypyridine | Leucaena leucocephala | 3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate | - |
? | |
O3-acetyl-L-serine + N-hydroxyurea | 13% of the activity with 3,4-dihydroxypyridine | Leucaena leucocephala | ureidoserine + acetate | - |
? | |
O3-acetyl-L-serine + pyrazole | 82% of the activity with 3,4-dihydroxypyridine | Leucaena leucocephala | 3-(pyrazol-1-yl)-L-alanine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Leucaena leucocephala |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
reaction with 3,4-dihydroxypyridine | Leucaena leucocephala |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit | Leucaena leucocephala |