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Literature summary for 2.5.1.5 extracted from

  • Qin, X.; Ma, C.; Lou, Z.; Wang, H.
    Effects of additives on the lyophilized and thermal stability of D-galactose-6-sulfurylase activity from Eucheuma striatum (Rhodophyta) (2015), J. Appl. Phycol., 27, 1709-1715.
No PubMed abstract available

General Stability

General Stability Organism
the addition of 5% (w/v) sucrose and trehalose prior to lyophilization can retain nearly 100% enzymatic activity, compared to 19% for the lyophilized control. Polyol sucrose and amino acid glycine enhance the thermal stability of the enzyme, while polyol mannitol decreases the thermal stability Kappaphycus striatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mu-carrageenan Kappaphycus striatus
-
sulfate + kappa-carrageenan
-
?
ni-carrageenan Kappaphycus striatus
-
sulfate + iota-carrageenan
-
?
porphyran Kappaphycus striatus
-
sulfate + agarose
-
?

Organism

Organism UniProt Comment Textmining
Kappaphycus striatus
-
-
-

Storage Stability

Storage Stability Organism
-20°C, lyophilized enzyme, 40 days, no loss of activity Kappaphycus striatus
-20°C, with glycerol, 20 days, 20% loss of activity Kappaphycus striatus
4°C, crude extract at pH 5.0, 1 day, more than 60% loss of activity Kappaphycus striatus
4°C, crude extract at pH 7.0 with 10 mM beta-mercaptoethanol, 7 days, no loss of activity Kappaphycus striatus
4°C, crude extract at pH 7.0, 7 days, 45% loss of activity Kappaphycus striatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mu-carrageenan
-
Kappaphycus striatus sulfate + kappa-carrageenan
-
?
ni-carrageenan
-
Kappaphycus striatus sulfate + iota-carrageenan
-
?
porphyran
-
Kappaphycus striatus sulfate + agarose
-
?

Synonyms

Synonyms Comment Organism
D-galactose-6-sulfurylase
-
Kappaphycus striatus
Gal-6-sulfurylase
-
Kappaphycus striatus