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Literature summary for 2.5.1.49 extracted from

  • Bolten, C.J.; Schroeder, H.; Dickschat, J.; Wittmann, C.
    Towards methionine overproduction in Corynebacterium glutamicum - methanethiol and dimethyldisulfide as reduced sulfur sources (2010), J. Microbiol. Biotechnol., 20, 1196-1203.
    View publication on PubMed
Search for more literature for 2.5.1.49

Application

Application Comment Organism
synthesis overexpression of gene metY increases the in vitro activity of O-acetylhomoserine aminocarboxypropyltransferase to 1780 mU/mg and is beneficial for methionine production, since the intracellular methionine pool increases 2fold in the engineered strain Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
61
-
 substrate dimethyldisulfide, pH 7.8, 30°C Corynebacterium glutamicum
63
-
 substrate methanethiol, pH 7.8, 30°C Corynebacterium glutamicum
480
-
 substrate H2S, pH 7.8, 30°C Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 O-acetyl-L-homoserine + dimethyldisulfide dimethyldisulfide may be reductively cleaved into methanethiol prior to assimilation Corynebacterium glutamicum 2 L-methionine + 2 acetate
-
 ?
2 O-acetyl-L-homoserine + H2S
-
 Corynebacterium glutamicum 2 L-homocysteine + 2 acetate
-
 ?
O-acetyl-L-homoserine + methanethiol
-
 Corynebacterium glutamicum L-methionine + acetate
-
 ?

General Information

General Information Comment Organism
physiological function wild-type cells are able to grow on both methanethiol and dimethyldisulfide as sole sulfur sources. Both sulfur compounds are incorporated as an entire molecule, adding the terminal S-CH3 group to O-acetylhomoserine. In this reaction, methionine is directly formed. the reaction is catalyzed by O-acetylhomoserine sulfhydrylase MetY. The deletion of metY results in methionine auxotrophic strains grown on methanethiol or dimethyldisulfide as sole sulfur sources. In vitro studies with the wild-type reveal a relatively low activity of MetY for methanethiol and dimethyldisulfide Corynebacterium glutamicum