Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-propargylglycine | 89% inhibition at 1 mM | Thermus thermophilus | |
hydroxylamine | 95% inhibition at 1 mM, 76% at 0.1 mM | Thermus thermophilus | |
L-methionine | 68% inhibition at 1 mM | Thermus thermophilus | |
phenylhydrazine | 95% inhibition at 10 mM, 12% at 1 mM | Thermus thermophilus | |
Semicarbazide | 76% inhibition at 10 mM, 12% at 1 mM | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
O-acetyl-L-homoserine | at a fixed sulfide concentration of 1 mM | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
SDS-PAGE | Thermus thermophilus |
44160 | - |
calculated from amino acid sequence | Thermus thermophilus |
190000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q76K51 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q76K51 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.17 | - |
crude extract | Thermus thermophilus |
14.3 | - |
after heat treatment at 65°C | Thermus thermophilus |
36 | - |
Q Sepharose pool | Thermus thermophilus |
67.7 | - |
phenyl Sepharose pool | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + H2S | lesser activity | Thermus thermophilus | L-homocysteine + H2O | - |
? | |
L-homoserine + H2S | lesser activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-homocysteine + H2O | - |
? | |
L-serine + H2S | lesser activity | Thermus thermophilus | L-cysteine + H2O | - |
? | |
L-serine + H2S | lesser activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-cysteine + H2O | - |
? | |
o-acetyl-L-homoserine + H2S | - |
Thermus thermophilus | L-homocysteine + acetate | - |
? | |
o-acetyl-L-homoserine + H2S | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-homocysteine + acetate | - |
? | |
O-acetyl-L-serine | lesser activity | Thermus thermophilus | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine | lesser activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-cysteine + acetate | - |
? | |
O-phosphoryl-L-serine + H2S | lesser activity | Thermus thermophilus | L-cysteine + H2O | - |
? | |
O-succinyl-L-homoserine + H2S | lesser activity | Thermus thermophilus | L-homocysteine + succinate | - |
? | |
O-succinyl-L-homoserine + H2S | lesser activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-homocysteine + succinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Thermus thermophilus |
tetramer | 4 * 44000, SDS-PAGE, gel filtration | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
EC 4.2.99.10 | formerly | Thermus thermophilus |
o-acetyl-L-homoserine sulfhydrylase | - |
Thermus thermophilus |
OAH SHase | - |
Thermus thermophilus |
oah2 gene product | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
80% activity remaining at 90°C for 60 min | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 8.8 | less than 50% activity above and below | Thermus thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | - |
Thermus thermophilus |