General Stability | Organism |
---|---|
existence of glycerol in the purification buffer keeps the enzyme very stable, sensitive to salts such as (NH4)2SO4 and NaCl in absence of glycerol | Aspergillus nidulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-C-propagylglycine | only 25% inhibition | Aspergillus nidulans | |
L-methionine | only 20% inhibition | Aspergillus nidulans | |
additional information | no sulfhydryl reagents inhibits the enzyme, S-adenosylmethionine is no inhibitor | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
6 * 43000, SDS-PAGE | Aspergillus nidulans |
250000 | 260000 | gel filtration | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-homoserine + H2S | Aspergillus nidulans | - |
L-homocysteine + acetic acid | - |
? | |
O-acetyl-L-homoserine + H2S | Aspergillus nidulans sG8 | - |
L-homocysteine + acetic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
and strains pabaA2, YA1 | - |
Aspergillus nidulans sG8 | - |
and strains pabaA2, YA1 | - |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus nidulans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus nidulans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.96 | - |
- |
Aspergillus nidulans |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 6 months | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-homoserine + H2S | - |
Aspergillus nidulans | L-homocysteine + acetic acid | - |
? | |
O-acetyl-L-homoserine + H2S | - |
Aspergillus nidulans sG8 | L-homocysteine + acetic acid | - |
? | |
O-acetyl-L-serine + H2S | - |
Aspergillus nidulans | L-cysteine + acetic acid | - |
? | |
O-acetyl-L-serine + H2S | - |
Aspergillus nidulans sG8 | L-cysteine + acetic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 43000, SDS-PAGE | Aspergillus nidulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable at | Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
O-acetyl-L-serine as substrate | Aspergillus nidulans |
8 | - |
O-acetyl-L-homoserine as substrate | Aspergillus nidulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Aspergillus nidulans |