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Literature summary for 2.5.1.49 extracted from

  • Yamagata, S.; Paszewski, A.; Lewandowska, I.
    Purification and properties of O-acetyl-L-homoserine O-sulfhydrylase from Aspergillus nidulans (1990), J. Gen. Appl. Microbiol., 36, 137-141.
No PubMed abstract available

General Stability

General Stability Organism
existence of glycerol in the purification buffer keeps the enzyme very stable, sensitive to salts such as (NH4)2SO4 and NaCl in absence of glycerol Aspergillus nidulans

Inhibitors

Inhibitors Comment Organism Structure
DL-C-propagylglycine only 25% inhibition Aspergillus nidulans
L-methionine only 20% inhibition Aspergillus nidulans
additional information no sulfhydryl reagents inhibits the enzyme, S-adenosylmethionine is no inhibitor Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
6 * 43000, SDS-PAGE Aspergillus nidulans
250000 260000 gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-acetyl-L-homoserine + H2S Aspergillus nidulans
-
L-homocysteine + acetic acid
-
?
O-acetyl-L-homoserine + H2S Aspergillus nidulans sG8
-
L-homocysteine + acetic acid
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
and strains pabaA2, YA1
-
Aspergillus nidulans sG8
-
and strains pabaA2, YA1
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus nidulans

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus nidulans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.96
-
-
Aspergillus nidulans

Storage Stability

Storage Stability Organism
-20°C, stable for at least 6 months Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-homoserine + H2S
-
Aspergillus nidulans L-homocysteine + acetic acid
-
?
O-acetyl-L-homoserine + H2S
-
Aspergillus nidulans sG8 L-homocysteine + acetic acid
-
?
O-acetyl-L-serine + H2S
-
Aspergillus nidulans L-cysteine + acetic acid
-
?
O-acetyl-L-serine + H2S
-
Aspergillus nidulans sG8 L-cysteine + acetic acid
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 43000, SDS-PAGE Aspergillus nidulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable at Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
O-acetyl-L-serine as substrate Aspergillus nidulans
8
-
O-acetyl-L-homoserine as substrate Aspergillus nidulans

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Aspergillus nidulans