General Stability | Organism |
---|---|
enzyme remains stable after purification, no dilution inactivation is observed | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-methionine | - |
Neurospora crassa | |
S-adenosyl-L-homocysteine | - |
Neurospora crassa | |
S-adenosyl-L-methionine | - |
Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
H2S | - |
Neurospora crassa | |
0.8 | - |
methyl-mercaptan | - |
Neurospora crassa | |
7 | - |
O-acetyl-L-homoserine | - |
Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-homoserine + H2S | Neurospora crassa | - |
L-homocysteine + acetic acid | - |
? | |
O-acetyl-L-homoserine + methanethiol | Neurospora crassa | pathway for formation of methionine, incorporation of sulfur, possible temporary function for this enzyme may be formation of methionine from methylmercaptan at times of exhaustion of an exogenous source of sulfur | L-methionine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
wild-type, strain EM 5297 | - |
Purification (Comment) | Organism |
---|---|
- |
Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
- |
Neurospora crassa |
4.2 | - |
O-acetylhomoserine sulfhydrylation | Neurospora crassa |
7.7 | - |
O-acetylhomoserine methylsulfhydrylation | Neurospora crassa |
Storage Stability | Organism |
---|---|
-15°C, 80% of the activity remains after storage for 6 months | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no cystathione formed from cysteine, no alpha-ketobutyrate formed from O-acetylhomoserine, negligible activity with homoserine and serine, O-succinylhomoserine, O-phosphorylhomoserine, O-acetylserine and O-phosphorylserine are no substrates | Neurospora crassa | ? | - |
? | |
O-acetyl-L-homoserine + H2S | - |
Neurospora crassa | L-homocysteine + acetic acid | - |
? | |
O-acetyl-L-homoserine + methanethiol | - |
Neurospora crassa | L-methionine + acetic acid | - |
? | |
O-acetyl-L-homoserine + methanethiol | pathway for formation of methionine, incorporation of sulfur, possible temporary function for this enzyme may be formation of methionine from methylmercaptan at times of exhaustion of an exogenous source of sulfur | Neurospora crassa | L-methionine + acetate | - |
? | |
O-acetyl-L-homoserine + methyl mercaptan | - |
Neurospora crassa | L-methionine + acetic acid | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.5 | 50% activity at pH 6.6, 90% activity at pH 7.5 and 8.5 | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Neurospora crassa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
S-adenosylmethionine | - |
Neurospora crassa |