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Literature summary for 2.5.1.49 extracted from
- O-Acetylhomoserine sulfhydrylase (Neurospora) (1971), Methods Enzymol., 17, 446-450.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| enzyme appears to be quite stable | Neurospora crassa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no end product inhibition by methionine or S-adenosylmethionine observed | Neurospora crassa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
H2S | - |
Neurospora crassa |  |
| 0.8 | - |
methyl-mercaptan | - |
Neurospora crassa | |
| 7 | - |
O-acetyl-L-homoserine | - |
Neurospora crassa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-homoserine + H2S | Neurospora crassa | - |
L-homocysteine + acetic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 80% of the activity remains after storage for 6 months | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | L-homoserine, O-succinyl-DL-homoserine, O-phosphoryl-L-homoserine, L-serine, O-acetyl-L-serine, O-phosphoryl-L-serine react at less than 1% the rate with O-acetyl-L-homoserine | Neurospora crassa | ? | - |
? | |
| O-acetyl-L-homoserine + H2S | - |
Neurospora crassa | L-homocysteine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + methyl mercaptan | - |
Neurospora crassa | L-methionine + acetic acid | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | only required after enzyme is resolved by dialysis against cysteine | Neurospora crassa | |
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Release 2023.1 (January 2023)
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