Protein Variants | Comment | Organism |
---|---|---|
D45F | reduction in catalytic effciency, without change in reaction specificity | Escherichia coli |
D45F/E325Y | reduction in catalytic effciency, without change in reaction specificity | Escherichia coli |
E325Y | reduction in catalytic effciency, without change in reaction specificity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
L-cystathionine | wild-type, 25°C, pH 7.8 | Escherichia coli | |
0.26 | - |
L-cystathionine | mutant E325Y 25°C, pH 7.8 | Escherichia coli | |
1.3 | - |
O-succinyl-L-homoserine | wild-type, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
1.5 | - |
O-succinyl-L-homoserine | mutant D45F/E325Y, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
1.7 | - |
O-succinyl-L-homoserine | mutant E325Y, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
2 | - |
O-succinyl-L-homoserine | mutant D45F, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
2.1 | - |
O-succinyl-L-homoserine | mutant E325Y, gamma-replacement, 25°C, pH 7.8 | Escherichia coli | |
2.4 | - |
L-cystathionine | mutant D45F, 25°C, pH 7.8 | Escherichia coli | |
2.5 | - |
O-succinyl-L-homoserine | wild-type, gamma-replacement, 25°C, pH 7.8 | Escherichia coli | |
5.4 | - |
O-succinyl-L-homoserine | mutant D45F, gamma-replacement, 25°C, pH 7.8 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + acetate | - |
Escherichia coli | O-acetyl-L-homoserine + L-cysteine | - |
r | |
O-acetyl-L-homoserine + L-cysteine | gamma-replacement | Escherichia coli | L-cystathionine + acetate | - |
r | |
O-succinyl-L-homoserine + H2O | gamma-elimination | Escherichia coli | succinate + 2-oxobutyrate + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.23 | - |
O-succinyl-L-homoserine | mutant D45F/E325Y, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
1.39 | - |
O-succinyl-L-homoserine | mutant E325Y, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
1.4 | - |
O-succinyl-L-homoserine | mutant D45F, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
1.8 | - |
O-succinyl-L-homoserine | wild-type, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
5.6 | - |
L-cystathionine | mutant D45F, 25°C, pH 7.8 | Escherichia coli | |
11.1 | - |
L-cystathionine | mutant E325Y 25°C, pH 7.8 | Escherichia coli | |
121 | - |
L-cystathionine | wild-type, 25°C, pH 7.8 | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.69 | - |
O-succinyl-L-homoserine | mutant D45F, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
0.8 | - |
O-succinyl-L-homoserine | mutant D45F/E325Y, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
0.8 | - |
O-succinyl-L-homoserine | mutant E325Y, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
1.35 | - |
O-succinyl-L-homoserine | wild-type, gamma-elimination, 25°C, pH 7.8 | Escherichia coli | |
2 | - |
L-cystathionine | mutant D45F, 25°C, pH 7.8 | Escherichia coli | |
3 | - |
O-succinyl-L-homoserine | mutant D45F, gamma-replacement, 25°C, pH 7.8 | Escherichia coli | |
7 | - |
O-succinyl-L-homoserine | mutant E325Y, gamma-replacement, 25°C, pH 7.8 | Escherichia coli | |
41 | - |
L-cystathionine | mutant E325Y 25°C, pH 7.8 | Escherichia coli | |
49 | - |
O-succinyl-L-homoserine | wild-type, gamma-replacement, 25°C, pH 7.8 | Escherichia coli | |
1060 | - |
L-cystathionine | wild-type, 25°C, pH 7.8 | Escherichia coli |