Literature summary for 2.5.1.48 extracted from

Clausen, T.; Wahl, M.C.; Messerschmidt, A.; Huber, R.; Fuhrmann, J.C.; Laber, B.; Streber, W.; Steegborn, C.
Cloning, purification, and characterization of cystathionine g-synthase from Nicotiana tabacum (1999), Biol. Chem., 380, 1237-1242.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Nicotiana tabacum

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Nicotiana tabacum

Inhibitors

Inhibitors	Comment	Organism	Structure
(Z)-3-(2-phosphonoethen-1-yl)pyridine-2-carboxylic acid	-	Nicotiana tabacum
3-(phosphonomethyl)pyridine-2-carboxylic acid	-	Nicotiana tabacum
4-(phosphonomethyl)pyridine-2-carboxylic acid	-	Nicotiana tabacum
DL-(E)-2-amino-5-phosphono-3-pentenoic acid	-	Nicotiana tabacum
DL-propargylglycine	irreversible	Nicotiana tabacum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.23	-	L-cysteine	-	Nicotiana tabacum
0.55	-	Sodium sulfide	-	Nicotiana tabacum
0.89	-	L-cysteine methyl ester	-	Nicotiana tabacum
2.6	-	2-mercaptoethanol	-	Nicotiana tabacum
7.1	-	O-phospho-L-homoserine	-	Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
O-succinyl-L-homoserine + L-cysteine	Nicotiana tabacum	the enzyme catalyzes the first reaction specific for methionine biosynthesis	L-cystathionine + succinate	-	?

Organism

Organism	UniProt	Comment	Textmining
Nicotiana tabacum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Nicotiana tabacum

Reaction

Reaction	Comment	Organism	Reaction ID
O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate	ping-pong mechanism	Nicotiana tabacum	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
O-acetyl-L-homoserine + L-Cys methyl ester	26% of the activity with L-Cys	Nicotiana tabacum	S-(L-2-amino-2-methoxycarbonyl)-L-homocysteine + acetate	-	?
O-phospho-L-homoserine + L-cysteine	-	Nicotiana tabacum	L-cystathionine + phosphate	-	?
O-succinyl-L-homoserine + 2-mercaptoethanol	32% of the activity with L-cysteine	Nicotiana tabacum	S-ethyl-L-homocysteine + succinate	-	?
O-succinyl-L-homoserine + D-cysteine	20% of the activity with L-cysteine	Nicotiana tabacum	?	-	?
O-succinyl-L-homoserine + L-cysteine	the enzyme catalyzes the first reaction specific for methionine biosynthesis	Nicotiana tabacum	L-cystathionine + succinate	-	?
O-succinyl-L-homoserine + sodium sulfide	62% of the activity with L-cysteine	Nicotiana tabacum	L-homocysteine + sodium succinate	-	?

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	enzyme is dependent on	Nicotiana tabacum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.018	-	DL-propargylglycine	-	Nicotiana tabacum
0.027	-	DL-(E)-2-amino-5-phosphono-3-pentenoic acid	-	Nicotiana tabacum
0.2	-	3-(phosphonomethyl)pyridine-2-carboxylic acid	-	Nicotiana tabacum
0.3	-	4-(phosphonomethyl)pyridine-2-carboxylic acid	-	Nicotiana tabacum
0.45	-	(Z)-3-(2-phosphonoethen-1-yl)pyridine-2-carboxylic acid	-	Nicotiana tabacum

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Release 2023.1 (January 2023)