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Literature summary for 2.5.1.48 extracted from

  • Ravanel, S.; Droux, M.; Douce, R.
    Methionine biosynthesis in higher plants. I. Purification and characterization of cystathionine gamma-synthase from spinach chloroplasts (1995), Arch. Biochem. Biophys., 316, 572-584.
    View publication on PubMed
Search for more literature for 2.5.1.48

Inhibitors

Inhibitors Comment Organism Structure
propargylglycine
-
 Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 L-cysteine pH 7.5, 30°C Spinacia oleracea
0.6
-
 S2- pH 7.5, 30°C Spinacia oleracea
1.4
-
 O-phospho-L-homoserine pH 7.5, 30°C Spinacia oleracea
13.34
-
 O-succinyl-L-homoserine pH 7.5, 30°C Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Spinacia oleracea 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000 or 2 * 50000 + 2 * 53000, SDS-PAGE Spinacia oleracea
53000
-
 4 * 50000 or 2 * 50000 + 2 * 53000, SDS-PAGE Spinacia oleracea
215000
-
 gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
 spinach
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the 50000 Da polypeptide may be derived from the 53000 Da polypeptide by proteolysis Spinacia oleracea

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.11
-
-
 Spinacia oleracea

Storage Stability

Storage Stability Organism
-20°C, 20 mM MOPS-NaOH, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% v/v glycerol, 5 days, 50% loss of activity Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + H2O
-
 Spinacia oleracea L-homocysteine + NH3 + pyruvate
-
 ?
O-phospho-L-homoserine + L-cysteine
-
 Spinacia oleracea L-cystathionine + phosphate
-
 ?
O-phospho-L-homoserine + S2-
-
 Spinacia oleracea L-homocysteine + phosphate
-
 ?
O-succinyl-L-homoserine + L-cysteine
-
 Spinacia oleracea L-cystathionine + succinate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000 or 2 * 50000 + 2 * 53000, SDS-PAGE Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Spinacia oleracea