Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Methyl-2-benzothiazolinone hydrazone | - |
Lysinibacillus sphaericus | |
D-cycloserine | - |
Lysinibacillus sphaericus | |
hydroxylamine | - |
Lysinibacillus sphaericus | |
additional information | not inhibited by p-chloromercuribenzoate, iodoacetate, N-ethylmaleimide, 5,5'-dithiobis(2-nitrobenzoate), NaCN | Lysinibacillus sphaericus | |
phenylhydrazine | - |
Lysinibacillus sphaericus | |
Semicarbazide | - |
Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Lysinibacillus sphaericus |
165000 | - |
gel filtration | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
118 | - |
- |
Lysinibacillus sphaericus |
Storage Stability | Organism |
---|---|
-20°C, 10 mM potassium phosphate buffer, pH 7.5, 50% glycerol | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | beta-elimination and gamma-elimination barely catalyzed | Lysinibacillus sphaericus | ? | - |
? | |
O-acetyl-L-homoserine + 2-mercaptoethanol | 150% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-hydroxyethyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + 2-mercaptopropionate | 50% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-methylcarboxymethyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + 3-mercaptopropionate | 65% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-carboxyethyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + allyl mercaptan | 4% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-allyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + benzyl mercaptan | 41% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-benzyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + CH3SH | 11% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | L-methionine + acetate | - |
? | |
O-acetyl-L-homoserine + D-Cys | 101% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | D-allocystathionine + acetate | - |
? | |
O-acetyl-L-homoserine + D-homocysteine | 125% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | meso-homolanthionine + acetate | - |
? | |
O-acetyl-L-homoserine + ethyl mercaptan | 24% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | L-thionine + acetate | - |
? | |
O-acetyl-L-homoserine + isobutyl mercaptan | 13% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-isobutyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + isopropyl mercaptan | 1% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-isopropyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + L-Cys ethyl ester | 15% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-[(2R)-2-amino-3-ethoxy-3-oxopropyl]-L-homocysteine + acetate | i.e. L-cystathionine monoethyl ester + acetate | ? | |
O-acetyl-L-homoserine + L-Cys methyl ester | 20% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-(L-2-amino-2-methoxycarbonyl)-L-homocysteine + acetate | i.e. L-cystathionine monomethyl ester + acetate | ? | |
O-acetyl-L-homoserine + L-cysteine | - |
Lysinibacillus sphaericus | L-cystathionine + acetate | - |
? | |
O-acetyl-L-homoserine + L-homocysteine | 92% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | L-homolanthionine + acetate | - |
? | |
O-acetyl-L-homoserine + m-thiocresol | 150% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-m-tolyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + N-acetyl-L-Cys | 12% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-(L-acetylamino-2-carboxyethyl)-L-homocysteine + acetate | i.e. mono-N-acetyl-L-cystathionine + acetate | ? | |
O-acetyl-L-homoserine + n-butyl mercaptan | 5% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-n-butyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + n-propyl mercaptan | 13% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-n-propyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + o-thiocresol | 3% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-o-tolyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + p-nitrothiophenol | 41% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-p-nitrophenyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + p-thiocresol | 25% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-p-tolyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + phenyl mercaptan | 98% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-phenyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + S2- | 163% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + sec-butyl mercaptan | 5% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-sec-butyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + tert-butyl mercaptan | 2% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-tert-butyl-L-homosysteine + acetate | - |
? | |
O-acetyl-L-homoserine + thioacetic acid | 3% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-acetyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + thioglycolate ethyl ester | 348% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-ethoxycarbonyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + thioglycolate n-butyl ester | 274% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-n-butoxycarbonyl-L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + thiosalicylic acid | 63% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | S-o-carboxyphenyl-L-homocysteine + acetate | - |
? | |
O-malonyl-L-homoserine + L-cysteine | 14% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | L-cystathionine + malonate | - |
? | |
O-phenyl-L-homoserine + L-cysteine | 1% relative activity to O-acetyl-L-homoserine + L-cysteine | Lysinibacillus sphaericus | L-cystathionine + phenol | - |
? | |
O-succinyl-L-homoserine + L-cysteine | - |
Lysinibacillus sphaericus | L-cystathionine + succinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, SDS-PAGE | Lysinibacillus sphaericus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 4 mol per mol of enzyme, Km: 0.021 mM | Lysinibacillus sphaericus |