Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a rational drug target against amebiasis | Entamoeba histolytica |
Cloned (Comment) | Organism |
---|---|
gene CS1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Entamoeba histolytica |
gene CS3, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aggreticin | - |
Entamoeba histolytica | |
deacetylkinamycin C | - |
Entamoeba histolytica | |
deoxyfrenolicin | - |
Entamoeba histolytica | |
exophillic acid | from Exophiala sp.FKI-7082 , specific for isozyme CS1 | Entamoeba histolytica | |
kerriamycin B | - |
Entamoeba histolytica | |
kerriamycin C | - |
Entamoeba histolytica | |
additional information | identification and evaluation of natural inhibitors of Entamoeba histolytica cysteine synthase from microbial secondary metabolites, high-throughput screening. Terreinol and citromycetin are poor inhibitors; identification and evaluation of natural inhibitors of Entamoeba histolytica cysteine synthase from microbial secondary metabolites, high-throughput screening. Terreinol and citromycetin are poor inhibitors, no inhibition of isozyme CS3 by exophillic acid from Exophiala sp.FKI-7082, which is specific for isozyme CS1 | Entamoeba histolytica | |
nanaomycin A | - |
Entamoeba histolytica | |
naphthacemycin A | - |
Entamoeba histolytica | |
patulin | - |
Entamoeba histolytica | |
tetracycline | - |
Entamoeba histolytica | |
xanthofulvin | from Penicillium sp. if08054; from Penicillium sp. if08054 , inhibits isozymes CS1 and CS3 | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | Entamoeba histolytica | - |
L-cysteine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | O15635 | - |
- |
Entamoeba histolytica | Q401L7 | - |
- |
no activity in Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | - |
Entamoeba histolytica | L-cysteine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CS1 | - |
Entamoeba histolytica |
CS3 | - |
Entamoeba histolytica |
EhCS1 | - |
Entamoeba histolytica |
EhCS3 | - |
Entamoeba histolytica |
O-acetylserine sulfhydrylase | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Entamoeba histolytica |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00031 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | kerriamycin B | |
0.00056 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | kerriamycin C | |
0.00063 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | kerriamycin B | |
0.00064 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | aggreticin | |
0.00098 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | aggreticin | |
0.0012 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | kerriamycin C | |
0.021 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | deacetylkinamycin C | |
0.022 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | deacetylkinamycin C | |
0.053 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | nanaomycin A | |
0.053 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | deoxyfrenolicin | |
0.057 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | deoxyfrenolicin | |
0.065 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | nanaomycin A | |
0.075 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | naphthacemycin A | |
0.17 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | tetracycline | |
0.19 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | tetracycline | |
0.47 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | patulin | |
0.49 | - |
pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | patulin | |
0.49 | - |
above, pH 7.5, 37°C, recombinant enzyme | Entamoeba histolytica | naphthacemycin A |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is part of the sulfur assimilatory de novo L-cysteine biosynthetic pathway, that is essential for various cellular activities, including the proliferation and anti-oxidative defense of Entamoeba histolytica | Entamoeba histolytica |