Cloned (Comment) | Organism |
---|---|
recombinant expression of the enzyme under stress conditions in Trypanosoma rangeli, a trypanosome that does not perform cysteine biosynthesis de novo, results in rescue of the cysteine synthase activity and increased rates of survival of epimastigotes expressing the enzyme compared to those of wild-type parasites | Leishmania braziliensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | Leishmania braziliensis | - |
L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | Leishmania braziliensis MHOM/BR/75/M2904 | - |
L-cysteine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania braziliensis | A4HPM6 | - |
- |
Leishmania braziliensis MHOM/BR/75/M2904 | A4HPM6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | intracellular | Leishmania braziliensis | - |
additional information | quantitative enzyme expression analyis, overview | Leishmania braziliensis | - |
promastigote | - |
Leishmania braziliensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | - |
Leishmania braziliensis | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | - |
Leishmania braziliensis MHOM/BR/75/M2904 | L-cysteine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LbrCS | - |
Leishmania braziliensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leishmania braziliensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Leishmania braziliensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Leishmania braziliensis |
Organism | Comment | Expression |
---|---|---|
Leishmania braziliensis | the enzyme is upregulated under in vitro stress conditions induced by hydrogen peroxide, S-nitroso-N-acetylpenicillamine, or antimonial compounds in a developmental stage-specific manner | up |
General Information | Comment | Organism |
---|---|---|
physiological function | upregulation of cysteine synthase and cystathionine beta-synthase contributes to Leishmania braziliensis survival under oxidative stress. Ability of Leishmania braziliensis promastigotes and amastigotes overexpressing the enzyme to resist oxidative stress, which is significantly enhanced compared to that of nontransfected cells, resulting in a phenotype far more resistant to treatment with the pentavalent form of sodium stibogluconate in vitro | Leishmania braziliensis |