Crystallization (Comment) | Organism |
---|---|
to 1.8 A resolution. The biologically active unit, a dimer, constitutes the asymmetric unit. Subunit A contains residues 3-213 and 241-333, whilst subunit B comprises residues 4-214 and 241-333. A surface loop from residues 214 to 241 is disordered. The subunit contains two domains. The smaller domain I is constructed by residues 51-158, which primarily form a four-stranded beta-sheet surrounded by four alpha-helices. The larger domain II comprises residues 21-50 and 159-306. Domain II contains four alpha-helices and six beta-strands which, together with a beta-strand contributed from the partner-subunit domain I, form a seven-membered beta-sheet. In addition, residues 307-333 at the C-terminus form an extended helix-loop-helix structure that stretches across the surface of the partner subunit | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DYVI | a peptide based on the C-terminus of the partner serine acetyltransferase with which the enzyme forms a complex, competitive inhibition | Leishmania major |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35600 | - |
x * 35600, calculated | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
180 | - |
pH 7.8, 22°C | Leishmania major |
Subunits | Comment | Organism |
---|---|---|
? | x * 35600, calculated | Leishmania major |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | residue K51 forms a Schiff base with pyridoxal 5'-phosphate, with conserved residues N82 and S274 forming hydrogen bonds to the cofactor. Further residues predicted to orient and hold the pyridoxal 5'-phosphate in position are G186, T182, G183 and T185 | Leishmania major |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
DYVI | pH 7.8, 22°C | Leishmania major |