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Literature summary for 2.5.1.47 extracted from

  • Zhang, C.; Meng, Q.; Zhang, M.; Huang, F.; Gai, J.; Yu, D.
    Characterization of O-acetylserine(thiol)lyase-encoding genes reveals their distinct but cooperative expression in cysteine synthesis of soybean [Glycine max (L.) Merr.] (2008), Plant Mol. Biol. Rep., 26, 277-291.
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as GST-fusion proteins Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.062
-
 Sulfide
-
 Glycine max
0.063
-
 O-acetyl-L-serine
-
 Glycine max
0.105
-
 O-acetyl-L-serine
-
 Glycine max
0.11
-
 Sulfide
-
 Glycine max
0.116
-
 O-acetyl-L-serine
-
 Glycine max
0.355
-
 O-acetyl-L-serine
-
 Glycine max
0.53
-
 Sulfide
-
 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 calculated from cDNA Glycine max
60000
-
 calculated from cDNA Glycine max
66000
-
 calculated from cDNA Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max A3RM03
-
-
Glycine max A3RM04
-
-
Glycine max A3RM05
-
-
Glycine max A3RM06
-
-
Glycine max A5YT86
-
-
Glycine max A5YT88
-
-

Purification (Commentary)

Purification (Comment) Organism
using gel filtration Glycine max
using Glutathione Sepharose 4B column Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Glycine max
-
leaf
-
 Glycine max
-
root roots of seedlings Glycine max
-
seed
-
 Glycine max
-
seed mainly expressed in developing seeds Glycine max
-
stem young stems Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.262
-
-
 Glycine max
0.419
-
-
 Glycine max
0.489
-
-
 Glycine max
0.724
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine + sulfide
-
 Glycine max L-Cys + acetate
-
 ?

Synonyms

Synonyms Comment Organism
GmOAS-TL1
-
 Glycine max
GmOAS-TL2
-
 Glycine max
GmOAS-TL3
-
 Glycine max
GmOAS-TL4
-
 Glycine max
GmOAS-TL6
-
 Glycine max
GmOAS-TL7
-
 Glycine max

General Information

General Information Comment Organism
physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL1 grow in the M9 minimal medium in the absence of cysteine Glycine max
physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL2 does not grow in the M9 minimal medium in the absence of cysteine Glycine max
physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL3 grow in the M9 minimal medium in the absence of cysteine Glycine max
physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL4 grow in the M9 minimal medium in the absence of cysteine Glycine max
physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL6 grow in the M9 minimal medium in the absence of cysteine Glycine max
physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL7 does not grow in the M9 minimal medium in the absence of cysteine Glycine max