Any feedback?
Literature summary for 2.5.1.47 extracted from
- (31)P NMR studies of O-acetylserine sulfhydrylase-B from Salmonella typhimurium (2009), Arch. Biochem. Biophys., 487, 85-90.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-NTA column | Salmonella enterica subsp. enterica serovar Typhimurium |
| the apoenzyme of OASS-B is prepared using hydroxylamine as the resolving reagent. Apoenzyme is reconstituted to holoenzyme by addition of pyridoxal 5'-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium |
| using Ni-NTA chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
apo-enzyme reconstituted with cofactor pyridoxal 5'-phosphate exhibits 81.4% enzyme activity compared to the native enzyme, 100 mM HEPES, pH 7.0, with 0.2 mM O3-acetyl-L-serine, and 0.05 5-thio-2-nitrobenzoate | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 5 mM HEPES, pH 8.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-thio-2-nitrobenzoate + O-acetyl-L-serine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | acetate + ? | - |
? |  |
| O3-acetyl-L-serine + 5-thio-2-nitrobenzoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? + acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| O-acetylserine sulfhydrylase-B | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| OASS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| OASS-B | produced under unaerobic grwoth conditions in contrast to OASS-A | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay performed at room temperature | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | the 31P chemical shift of the internal and external Schiff bases of pyridoxal 5'-phosphate in OASS-B are further downfield compared to OASS-A, suggesting a tighter binding of the cofactor in the B-isozyme. The chemical shift of the internal Schiff base (ISB) of OASS-B is 6.2 ppm, the highest value reported for the ISB of a PLP-dependent enzyme. Considering the similarity in the binding sites of the pyridoxal 5'-phosphate cofactor for both isozymes, torsional strain of the C5-C5' bond (O4'-C5'-C5-C4) of the Schiff base is proposed to contribute to the further downfield shift | Salmonella enterica subsp. enterica serovar Typhimurium | |
| pyridoxal 5'-phosphate | tighter binding than in OASS-A, treatment with 1 mM hydroxylamine in 500 mM phosphate, pH 7.6, with 0.2 mM dithiothreitol, followed by dialysis against 100 mM phosphate, pH 7.0, with 0.2 mM dithiothreitol gerates apo-enzyme without the cofactor | Salmonella enterica subsp. enterica serovar Typhimurium | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
