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Literature summary for 2.5.1.47 extracted from
- Purification and characterization of a novel cysteine synthase isozyme from spinach hydrated seeds (1998), Biosci. Biotechnol. Biochem., 62, 501-507.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | 57% loss of activity at 10 mM, isoenzyme 1' | Spinacia oleracea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.66 | - |
H2S | pH 8.0, 25°, isoenzyme 1' | Spinacia oleracea | |
| 8.3 | - |
O-acetyl-L-Ser | pH 8.0, 25°, isoenzyme 1' | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | all isoenzymes | Spinacia oleracea | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
2 * 34000, SDS-PAGE | Spinacia oleracea |
| 68000 | - |
isoenzyme 1', gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
isoenzyme 1' | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | hydrated seeds | Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 834 | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-serine | - |
Spinacia oleracea | L-cysteine + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 34000, SDS-PAGE | Spinacia oleracea |
| More | isoenzyme 1', N-terminal amino acid sequence differs from those of enzymes one, two, and three | Spinacia oleracea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
1 min, no loss of activity | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
isoenzyme 1', lower than isoenzymes one, two, and three | Spinacia oleracea |
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Release 2023.1 (January 2023)
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