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Literature summary for 2.5.1.47 extracted from

  • Ikegami, F.; Kaneko, M.; Lambein, F.; Kuo, Y.H.; Murakoshi, I.
    Difference between uracilylalanine synthases and cysteine synthases in Pisum sativum (1987), Phytochemistry, 26, 2699-2704.
No PubMed abstract available
Search for more literature for 2.5.1.47

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 H2S pH 7.8, 35°C Pisum sativum
0.038
-
 H2S pH 7.8, 35°C, isoenzyme 2 Pisum sativum
2.1
-
 O-acetyl-L-Ser pH 8.0, 30°C, isoenzyme 1 Pisum sativum
2.3
-
 O-acetyl-L-Ser pH 8.0, 30°C, isoenzyme 2 Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, SDS-PAGE Pisum sativum
52000
-
 gel filtration Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 three isoforms
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seedling maximal activity Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
852
-
 isoenzyme 2 Pisum sativum
933
-
 isoenzyme 1 Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-Ser + 2-propene-1-thiol 2.6% and 6.8% of the activity with sulfide, isoenzyme 1 and 2, respectively Pisum sativum S-allyl-L-cysteine + ?
-
 ?
O-acetyl-L-Ser + H2S
-
 Pisum sativum L-Cys + acetate
-
 ?
O-acetyl-L-Ser + mercaptoacetic acid 2.3% and 1.6% of activity with sulfide, isoenzymes 1 and 2, respectively Pisum sativum S-carboxymethyl-L-cysteine
-
 ?
O-acetyl-L-Ser + methyl mercaptan 4% and 1% of activity with sulfide, isoenzymes 1 and 2, respectively Pisum sativum S-methylcysteine + acetate
-
 ?
O-acetyl-L-serine
-
 Pisum sativum L-cysteine + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Pisum sativum
More amino acid composition Pisum sativum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pisum sativum