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Literature summary for 2.5.1.47 extracted from

  • Yamagata, S.; Takeshima, K.; Naiki, N.
    Evidence for the identity of O-acetylserine sulfhydrylase with O-acetylhomoserine sulfhydrylase in yeast (1974), J. Biochem., 75, 1221-1229.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.12
-
 O-acetyl-L-Ser pH 7.8 Saccharomyces cerevisiae
6.67
-
 O-acetylhomoserine
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-acetyl-L-Ser + sulfide Saccharomyces cerevisiae key role in metabolism of S-containing amino acids L-Cys + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity buffer-dependent, substrate unstable in Tris-HCl buffer Saccharomyces cerevisiae
1.02
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-Ser + sulfide key role in metabolism of S-containing amino acids Saccharomyces cerevisiae L-Cys + acetate
-
 ?
O-acetylhomoserine + H2S
-
 Saccharomyces cerevisiae homocysteine + ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below Saccharomyces cerevisiae
65
-
 5 min, complete loss of activity Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
 Saccharomyces cerevisiae