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Literature summary for 2.5.1.47 extracted from

  • Droux, M.; Ruffet, M.L.; Douce, R.; Job, D.
    Interactions between serine acetyltransferase and O-acetylserine (thiol) lyase in higher plants. Structural and kinetic properties of the free and bound enzymes (1998), Eur. J. Biochem., 255, 235-245.
    View publication on PubMed
Search for more literature for 2.5.1.47

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 H2S pH 7.0, 25°C, recombinant free enzyme Arabidopsis thaliana
0.55
-
 H2S pH 7.5, 25°C, recombinant complex-bound enzyme Arabidopsis thaliana
0.64
-
 O-acetyl-L-Ser pH 7.5, 25°C, recombinant free enzyme Arabidopsis thaliana
4.28
-
 O-acetyl-L-Ser pH 7.5, 25°C, recombinant complex-bound enzyme Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast bienzyme complex Arabidopsis thaliana 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000 Arabidopsis thaliana
300000
-
 bienzyme complex with serine acetyltransferase, gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity modified by protein-protein interactions within cystein synthase complex Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine
-
 Arabidopsis thaliana L-cysteine + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36000 Arabidopsis thaliana
More two dimeric enzyme molecules form complex with tetrameric serine acetyltransferase, complex does not dissociate during catalysis Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 4 per cysteine synthase complex Arabidopsis thaliana