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Literature summary for 2.5.1.46 extracted from
- The hypusine cascade promotes cancer progression and metastasis through the regulation of RhoA in squamous cell carcinoma (2016), Oncogene, 35, 5304-5316.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DHPS, DHPS is a metastasis-associated gene within the specific amplification at chromosome 19p13.2p13.13 in HOC313-LM cells, quantitative RT-PCR expressionanalysis | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | DHPS gene silencing by transfection of DHPS-specific short hairpin RNA (shRNA) in LM-pGL4.5 cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N1-guanyl-1,7-diaminoheptane | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [eIF5A-precursor]-lysine + spermidine | Homo sapiens | - |
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P49366 | gene Dhps | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HOC313 cell | highly increased DHPS expression level | Homo sapiens | - |
| squamous cell carcinoma cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [eIF5A-precursor]-lysine + spermidine | - |
Homo sapiens | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHPS | - |
Homo sapiens |
| DHS | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | DHS enzyme inhibition by N1-guanyl-1,7-diaminoheptane results in a significant decrease in tumor formation in vivo. In patients with esophageal squamous cell carcinoma, overexpression of gene DHPS in ESCC tumors is significantly associated with worse recurrence-free survival, and correlated with distant metastasis. Although the mRNA levels of RhoA in DHPS-knockdown cells do not change, RhoA protein levels significantly decrease compared with control | Homo sapiens |
| physiological function | hypusine is formed from spermidine, a metabolite of polyamine metabolism, by the sequential action of two catalytic enzymes, deoxyhypusine synthase (DHPS) and deoxyhypusine hydroxylase (DOHH). These enzymes have an important role in post-translational modification of eukaryotic translation factor 5A (eIF5A), which functions in protein synthesis. The hypusine cascade contributes to cell proliferation and embryogenesis by the regulation of translation elongation in healthy cells. DHPS-mediated hypusine-modification of eukaryotic translation factor 5A facilitates the translation of RhoA, resulting in the activation of the RhoA signaling pathway and leading to not only increased cell motility, invasion and metastasis of cancer cells in vitro, but also increased tumor growth in vivo. DHPS regulates the expression and activation of RhoA, DHPS may regulate the organization of F-actin through the increase in RhoA protein level. Hypusinated eIF5A may regulate tumor growth and metastasis via the RhoA signaling pathway | Homo sapiens |
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Release 2023.1 (January 2023)
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